High Performance Liquid Chromatography
High Performance Liquid Chromatography (HPLC) and Ultra High Performance Liquid Chromatography (UHPLC) use liquid mobile phases to transport the sample components through a column packed with a solid material; the stationary phase. The "mode" of chromatography is usually defined by a combination of a certain stationary phase type with a certain mobile phase type. In order to generate a chromatogram that is suitable for qualitative or quantitative purposes, bonded phase chemistry, column dimensions, and particle and pore sizes are parameters that need to be optimized.
TSKgel Chromatography Columns
Tosoh Bioscience offers a comprehensive line of TSKgel (U)HPLC columns for use with conventional and UHPLC systems to meet your purification needs. The TSKgel product line include columns in nearly every mode of HPLC, including Size Exclusion, Ion Exchange, Reversed Phase, Hydrophobic Interaction, Affinity, and Normal Phase/HILIC.
Click on each mode listed below for a short tutorial on the mode, including an animated video, as well as the variety of TSKgel columns offered in that mode.
| Chromatography mode |
Types of Compounds analyzed |
| Size Exclusion Chromatography (SEC) » |
Natural (biopolymers), synthetic polymers |
| Ion Exchange Chromatography (IEC) » |
Charged compounds |
| Reversed Phase Chromatography (RPC) » |
Relatively hydrophobic compounds,
neutrals, weak acids, weak bases |
| Hydrophobic Interaction Chromatography (HIC) » |
Hydrophobic biomolecules |
| Hydrophilic Interaction Chromatography (HILIC) » |
Highly polar compounds |
| Affinity Chromatography(AFC) » |
Carbohydrates, proteins, specific anitgens |