SOLUTIONS

TSKgel Amide-80

TSKgel Amide-80

TSKgel Amide-80 columns are packed with 2, 3, 5, or 10 µm spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography.

TSKgel Amide-80 columns possess superior stability in aqueous/organic solvent systems, an advantage over other hydrophilic stationary phases. In addition, TSKgel Amide-80 columns are ideally suited for sensitive LC/MS analysis of water-soluble polar compounds because the high organic content in the mobile phase provides rapid evaporation of solvent during electrospray ionization.

Target applications for the TSKgel Amide-80 columns include the analysis of saccharides, glycans, oligosaccharides, peptides, and other polar compounds. The stationary phase does not react with reducing sugars and can be used at elevated temperatures (4 to 80 °C) to prevent peak splitting of carbohydrates that can occur at lower temperatures.

Comparison of TSKgel Amide-80 2 µm and 3 µm 

amide-80_fig1.png 

 

Comparing the retention of sugar alcohols 

amide-80_fig2.png 
Columns:        A: TSKgel Amide-80, 3 µm, 4.6 mm ID × 15 cm
                         B: Competitor Column, 4.6 mm ID × 15 cm
                         C: Competitor Column, 4.6 mm ID × 25 cm
                         D: Competitor Column, 4.6 mm ID × 25 cm
Mobile phase: H2O/ACN (25/75)
Flow rate:       1.0 mL/min
Detection:        RI
Temperature: 40 °C
Injection vol.: 10 µL
Samples:         1. ethylene glycol, 2. glycerin
                         3. erythritol, 4. xylitol
                         5. mannitol, 6. inositol 

 

Multiple Reaction Monitoring (MRM) chromatograms of milk and spiked milk samples - 10 ppb each 

amide-80_fig3.png 
Column:          TSKgel Amide-80, 3 µm, 2.0 mm ID × 15 cm
Mobile phase: A: 0.05% formic acid in H2O, B: 0.05% formic acid in ACN A/B = 25/75
Flow rate:        0.2 mL/min
Temperature: 40 °C
Injection vol.: 5 μL
Instrument:    Q TRAP (AB Sciex)
Ion source:      ESI
                         127/85+ (melamine)
                         128/42- (cyanuric acid)

 

Ordering Information

P/N Description Dimension
0021982 TSKgel Amide-80 HR, 5 µm 4.6 mm ID x 25 cm L
0023454 TSKgel Amide-80, 2 µm 2.0 mm ID x 5 cm L
0023455 TSKgel Amide-80, 2 µm 2.0 mm ID x 10 cm L
0023456 TSKgel Amide-80, 2 µm 2.0 mm ID x 15 cm L
0023457 TSKgel Amide-80, 2 µm 3.0 mm ID x 5 cm L
0023458 TSKgel Amide-80, 2 µm 3.0 mm ID x 10 cm L
0023459 TSKgel Amide-80, 2 µm 3.0 mm ID x 15 cm L
0021864 TSKgel Amide-80, 3µm 2.0 mm ID x 5 cm L
0021865 TSKgel Amide-80, 3µm 2.0 mm ID x 15 cm L
0022850 TSKgel Amide-80, 3 µm 3.0 mm ID x 5 cm L
0022851 TSKgel Amide-80, 3 µm 3.0 mm ID x 10 cm L
0022852 TSKgel Amide-80, 3 µm 3.0 mm ID x 15 cm L
0021866 TSKgel Amide-80, 3 µm 4.6 mm ID x 5 cm L
0021849 TSKgel Amide-80, 3 µm 4.6 mm ID x 10 cm L
0021867 TSKgel Amide-80, 3 µm 4.6 mm ID x 15 cm L
0020009 TSKgel Amide-80, 5 µm 1.0 mm ID x 5 cm L
0020010 TSKgel Amide-80, 5 µm 1.0 mm ID x 10 cm L
0021486 TSKgel Amide-80, 5 µm 1.0 mm ID x 15 cm L
0021487 TSKgel Amide-80, 5 µm 1.0 mm ID x 25 cm L
0019694 TSKgel Amide-80, 5 µm 2.0 mm ID x 5 cm L
0019695 TSKgel Amide-80, 5 µm 2.0 mm ID x 10 cm L
0019696 TSKgel Amide-80, 5 µm 2.0 mm ID x 15 cm L
0019697 TSKgel Amide-80, 5 µm 2.0 mm ID x 25 cm L
0019532 TSKgel Amide-80, 5 µm 4.6 mm ID x 5 cm L
0019533 TSKgel Amide-80, 5 µm 4.6 mm ID x 10 cm L
0013071 TSKgel Amide-80, 5 µm 4.6 mm ID x 25 cm L
0014459 TSKgel Amide-80, 10 µm 7.8 mm ID x 30 cm L
0014460 TSKgel Amide-80, 10 µm 21.5 mm ID x 30 cm L
0023460 TSKgel Amide-80 2 µm direct connect
Guardcolumn
2.0 mm ID x 1.0 cm L
0021862 TSKgel Amide-80 3 µm Guard Cartridge
for 2 mm ID Columns, 3 pieces/pkg
2.0 mm ID x 1.0 cm L
0019308 TSKgel Guard Cartridge Holder
for PN 0019325 & 0021941
2.0 mm ID x 1.0 cm L
0021863 TSKgel Amide-80 3 µm Guard Cartridge
for 4.6 mm ID Columns, 3 pieces/pkg
3.2 mm ID x 1.5 cm L
0019018 TSKgel Guard Cartridge Holder
for PN 0021863 & 0019010
3.2 mm ID x 1.5 cm L
0021941 TSKgel Amide-80 5 µm Guard Cartridge 
for 2 mm ID Columns, 3 pieces/pkg
2.0 mm ID x 1.0 cm L
0019021 TSKgel Amide-80 5 µm Guardcolumn 
for 4.6 mm ID Columns
4.6 mm ID x 1.0 cm L
0019010 TSKgel Amide-80 5 µm Guard Cartridge 
for 4.6 mm ID Columns, 3 pieces/pkg
3.2 mm ID x 1.5 cm L
0014461 TSKgel Amide-80 10µm Guardcolumn
for PN 14460
21.5 mm ID x 7.5 cm L