TSKgel Hydrophobic Interaction Chromatography Columns
The 2.5 μm nonporous methacrylate packing material of the TSKgel Butyl-NPR columns is bonded with butyl groups. These columns are the best choice for high speed separations with excellent recovery, even for more hydrophobic samples. As in other modes of liquid chromatography, smaller particles provide higher efficiency. Nonporous resin provides higher resolution at higher flow rates as well as excellent mass recovery, allowing quantitation down to nanogram levels.
Of the three TSKgel HIC columns, the TSKgel Ether-5PW columns have intermediate hydrophobicity. TSKgel Ether-5PW columns are stable in either acid or caustic cleaning regimens and provide excellent access to larger molecules with low diffusion coefficients.
The high porosity of TSKgel Phenyl-5PW packings allows very large proteins to enter the internal pore structure, thereby maintaining high capacity for such compounds.