TSKgel Butyl-NPR is based on a non-porous polymethacrylate base material with uniform 2.5 particle size. It is the least hydrophobic among the TSKgel HIC Columns and requires a higher salt concentration for binding.
TSKgel Butyl-NPR is the best choice for high speed separations with excellent recovery even for more hydrophobicity samples. The non-porous resin requires lower sample loading and leads to faster analysis time. TSKgel Butyl NPR is particularly popular for the analysis of drug to antibody ratios (DARs) of antibody drug conjugates (ADCs).
Separation of Fab and Fc fragments
|Column: TSKgel Butyl-NPR, 2.5 µm, 4.6 mm ID × 3.5 cm |
Mobile phase: A: 2 mol/L (NH4)2SO4, 20 mmol/L Tris, pH 7.0
B: 20 mmol/L Tris, pH 7.0
Gradient: 0 min (10%B) 34 min (100%B)
Flow rate: 1 mL/min
Temperature: 30 °C
Determination of Drug to Antibody Ratio using a TSKgel Butyl-NPR column
|Republished with permission of Genentech, Inc., from Analytical methods for physicochemical characterization of antibody drug conjugates, Wakankar A, Chen Y, Gokarn Y, Jacobson FS., 3, 2011; permission conveyed through Copyright Clearance Center, Inc. |