TSKgel DEAE-2SW
TSKgel DEAE-2SW columns are packed with 5 µm, porous, spherical silica beads which are surface modified with a weak anion exchange group. They are used for the separation and analysis of nucleotides, nucleosides, peptides, small proteins and other biologically active molecules. A 2 mm ID column is available for applications that are sample limited.
Separation of nucleotides
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Column: TSKgel DEAE-2SW, 5 µm, 4.6 mm ID × 25 cm
Mobile phase: A: CH3CN in 0.1 mol/L phosphate, pH 3.0, 20/80
B: CH3CN in 0.5 mol/L phosphate, pH 3.0, 20/80
Gradient: 30 min linear gradient from buffer A to B
Flow rate: 1.0 mL/min
Detection: UV @ 260 nm
Samples: 1. AMP 2. IMP 3. GMP 4. ADP 5. ATP |
Separation of phosphorothioates
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Column: TSKgel DEAE-2SW, 5 µm, 4.6 mm ID × 25 cm
Mobile phase: A: 50 mmol/L ammonium acetate
B: 1.5 mol/L ammonium acetate
Gradient: linear, 0-100%B in 60 minutes
Flow rate: 1 mL/min
Detection: UV @ 254 nm
Temperature: 25 °C
Samples: 1. 2 base phosphorothioate oligonucleotide
2. 3 base phosphorothioate oligonucleotide
3. 4 base phosphorothioate oligonucleotide
4. 5 base phosphorothioate oligonucleotide |