TSKgel Sugar AXG
The TSKgel Sugar AXG column is packed with 10 µm porous spherical polymer beads which are surface modified with a strong anion exchange group. They are used for the gradient separation and analysis of monosaccharides, disaccharides, and sugar alcohols.
For isocratic separation of carbohydrates where lower and constant back pressures may be generated, please see the TSKgel Sugar AXI column, which is packed with 8 µm particles.
Separation of saccharide mixture |
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Column: TSKgel Sugar AXG, 10 µm, 4. 6 mm ID × 15 cm
Mobile phase: step gradient: 6 min buffer A, 0.6 mol/L boric acid,
pH 7.7; then 27 min buffer B, 0.7 mol/L boric acid,
pH 7.25; then 30 min buffer C, 0.7 mol/L boric acid, pH 8.7
Flow rate: 0.4 mL/min (column and post column reagent solution)
Detection: fluorescence; Ex: 331 nm, Em: 383 nm
Pressure: 16 kg/cm2
Temperature: 70 ºC (column), 100 ºC (post column reactor)
PC reagent: 2.5% 2-cyanoacetamide solution
Samples: disaccharides, 25 nm; monosaccharides, 50 nm:
1. cellobiose 2. maltose 3. lactose
4. rhamnose 5. lyxose 6. ribose
7. mannose 8. fructose 9. arabinose
10. galactose 11. xylose 12. glucose |
Analysis of palatinit |
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Column: TSKgel Sugar AXG, 10 µm, 4.6 mm ID × 15 cm
Mobile phase: 0.7 mol/L borate buffer, pH 8.6
Flow rate: 0.8 mL/min
Detection: RI
Temperature: 65 ºC
Injection vol.: 10 µL
Samples: 1. alpha-D-glucopyranosyl-1,6-soribitol (GPS)
2. alpha-D-glucopyranosyl-1,6-mannitol (GPM) |