TSKgel Octadecyl-NPR
TSKgel Octadecyl-NPR is based on nonporous polymethacrylate particles of uniform 2.5 μm size, which provides high efficiency separations and fast analyses of peptides and proteins. The highly cross-linked polymethacrylate base material provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base.
TSKgel Octadecyl-NPR structure
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Analysis and recovery of nanogram protein samples
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Column: TSKgel Octadecyl-NPR, 2.5 µm, 4.6 mm ID × 3.5 cm
Mobile phase: 10 min linear gradient from 15% to 80% CH3CN in 5 mmol/L HCIO4
Flow rate: 1.5 mL/min
Detection: UV @ 220 nm
Samples: 50 ng each of 1. ribonuclease A, 2. insulin, 3. cytochrome C 4. lysozyme
5. transferrin, 6. myoglobin
Note: Blank gradient trace also shown |