TSKgel ODS-100Z
TSKgel ODS-100Z contains a high density monomeric C18 bonded phase with high ligand density (20% C) for maximum retention and selectivity of small molecular weight compounds. Exhaustive endcapping prevents secondary interaction with residual silanol groups.
This general purpose column is the workhorse for analysis of small molecular weight compounds in life science applications. TSKgel ODS-100Z columns are a great choice when a change of selectivity from the TSKgel ODS-100V columns is needed to resolve one or more overlapping peak pairs. Its excellent lot-to-lot reproducibility supports reversed phase applications in regulated environment.
Separation of tetracycline antibiotics
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Column: TSKgel ODS-100Z, 5 µm, 4.6 mm ID × 15 cm
Mobile phase: 10 mmol/L formic acid/ACN = 82.5/17.5
Flow rate: 1.0 mL/min
Detection: UV @ 254 nm
Temperature: 10 °C
Injection vol.: 20 μL
Samples: 1. tetracycline derivative, 2. oxytetracycline (20 mg/L),
3. tetracycline (20 mg/L), 4. doxycycline derivative,
5. chlortetracycline derivative, 6. chlortetracycline (30 mg/L),
7. doxycycline (30 mg/L) |
TSKgel ODS-100Z lot-to-lot variability
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Column: TSKgel ODS-100Z, 5 µm, 4.6 mm ID × 15 cm
Mobile phase: 20 mmol/L phosphate buffer, pH 7.0/MeOH = 20/80
Flow rate: 1.0 mL/min
Detection: UV @ 254 nm
Temperature: 40 °C
Injection vol.: 10 μL
Samples: 1. uracil, 2. toluene, 3. ethyl benzene, 4. quinizarin, 5. amitriptyline |