TSKgel ODS-140HTP
TSKgel ODS-140HTP columns provide high resolution and short analyses time at moderate pressures for high throughput separations. The polylayer bonding chemistry of the 2.3 µm particle results in highly efficient and durable columns. The moderate pressure drop compared to sub 2 µm columns reduces the burden on the hardware, allowing the TSKgel ODS-140HTP columns to be used with either UHPLC or conventional HPLC systems.
For use in high throughput applications, including drug discovery, pharmacokinetics and peptide digest separations, TSKgel ODS-140HTP columns offer excellent peak shape for basic compounds.
Durability of TSKgel ODS-140HTP columns
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Column: TSKgel ODS-140HTP, 2.3 µm, 2.1 mm ID × 10 cm L
Mobile phase: H2O/MeOH = 30/70
Flow rate: 0.2 mL/min
Detection: UV @ 254 nm
Temperature: 25 °C
Injection vol.: 2 μL
Samples: 1. phenol; 2. benzene; 3. toluene; 4. napthalene |
Analyses of six cold and sinus drug standards
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Column: TSKgel ODS-140HTP, 2.3 µm, 2.1 mm ID × 5 cm L
Mobile phase: A: H2O with 0.15% TFA; B: 100% ACN with 0.15% TFA
Gradient: Time (min) Solvent B (%) Flow (mL/min)
1.4 2.0 0.6
1.5 24.0 1.4
2.1 1.4
2.2 0.8
4.0 5.0 0.8
4.1 1.0 0.6
Detection: UV @ 215 nm
Temperature: 50 °C
Injection vol.: 10 μL
Samples: 1. maleate peak; 2. phenylephrine, 3. acetaminophen,
4. doxylamine succinate; 5. chlorpheniramine maleate;
6. dextromethorphan HBr; 7. diphenhydramine HCl |