TSKgel OligoDNA-RP
TSKgel OligoDNA-RP is specifically designed for the purification of oligonucleotides, and RNA and DNA fragments (up to 500-mer). TSKgel OligoDNA-RP columns can provide excellent separations of samples with very similar sequences. The packing is prepared by monomeric binding of octadecyl silyl groups to 5 μm spherical silica gel with 25 nm (250 Å) pores. This packing is not endcapped and it has a relatively low carbon content of 10%.
TSKgel OligoDNA-RP structure |
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Separation of octomers |
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Column: TSKgel OligoDNA, 4.6 mm ID x 5 cm
Mobile phase: 120 min linear gradient from 5% to 25% CH3CN
in 0.1mol/L ammonium acetate, pH7.0
Flow rate: 1.0 mL/min
Detection: UV @ 260 nm
Sample: 1. linker EcoR I, d(CGAATTCG)
2. Hpa I, d(CGTTAACG)
3. linker EcoR V, d(CGATATCG) |