TSKgel Phenyl-5PW RP
TSKgel Phenyl-5PW RP is based on a large pore, highly cross-linked polymethacrylate base matrix bonded with phenyl groups through an ether linkage. The polymeric matrix provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The large pore size of TSKgel Phenyl-5PW columns, 100 nm (1000Å), allows unhindered access to proteins and other large biopolymers.
The TSKgel Phenyl-5PW RP column is structurally similar to the TSKgel Phenyl-5PW column used in hydrophobic interaction chromatography (HIC), but the RP column packing is prepared by bonding a higher density of phenyl groups.
TSKgel Phenyl-5PW RP structure |
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Chemical Stability |
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Column: TSKgel Phenyl-5PW RP, 10 µm, 4.6 mm ID × 7.5 cm
Mobile phase: 60 min linear gradient from 5% to 80% CH3CN in 0.05% TFA
Flow rate: 1.0 mL/min
Detection: UV @ 220 nm
Samples: 10 µg each of
1. ribonuclease A, 2. cytochrome C
3. lysozyme, 4. bovine serum albumin
5. myoglobin |