SOLUTIONS

TSKgel Protein C4-300

TSKgel Protein C4-300

TSKgel Protein C4-300 columns are designed for the optimal recovery and resolution of proteins such as recombinant proteins, antibody fragments or PEGylated proteins. Its pore size of 30 nm (300 Å) is ideal for the separation of proteins. A particle size of 3 μm and optimized ligand density and alkyl length result in better protein and peptide resolution compared to other leading RP-C4 HPLC phases.

The C4 short alkyl chain ligand and its controlled bonding density provide moderate hydrophobicity to the stationary phase, which results in protein separations with high recovery and less peak tailing.

Comparison of standard protein separation 

proteinc4_fig1.png 
Columns:        A. TSKgel Protein C4-300, 3 µm, 4.6 mm ID × 15 cm L
                         B. Competitor A, 3.5 μm, 4.6 mm ID × 15 cm L
Mobile phase: A: H2O/CH3CN/TFA = 90/10/0.05 (v/v/v)
                         B: H2O/CH3CN/TFA = 20/80/0.05 (v/v/v)
Gradient:         0 min (0%B) 45 min (100% B)
Flow rate:       1.0 mL/min
Detection:       UV @ 210 nm
Temperature: 40 °C
Injection vol.: 10 μL
Samples:         1. cytochrome C; 2. lysozyme; 3. BSA; 4. α-chymotrypsinogen A;
                         5. ovalbumin (each 2 μg/10 μL)  

 

High speed separation of proteins 

proteinc4_fig2.png 
Column:              TSKgel Protein C4-300, 3 µm, 4.6 mm ID × 5 cm L
Mobile phase A: H2O/CH3CN/TFA = 90/10/0.05 (v/v/v)
Mobile phase B: H2O/CH3CN/TFA = 20/80/0.05 (v/v/v)
Gradient:             0 min (0% B) 5 min (100% B)
Flow rate:            3.0 mL/min
Detection:            UV @ 210 nm
Temperature:      40 °C
Injection vol.:      10 μL
Samples:              1. phenylalanine; 2. cytochrome C; 3. lysozyme;
                              4. BSA; 5. α-chymotrypsinogen A; 6. ovalbumin (each 0.2 g/μL)

 

Ordering Information

P/N Description Dimension
0022830 TSKgel Protein C4-300 2 mm ID x 5 cm L
0022831 TSKgel Protein C4-300 2 mm ID x 10 cm L
0022832 TSKgel Protein C4-300 2 mm ID x 15 cm L
0022834 TSKgel Guard Cartridges for 2.0 mm ID columns,
3 pieces/pkg
2 mm ID x 1 cm L
0019308 TSKgel Guard Cartridge holder 2 mm ID x 1 cm L
0022827 TSKgel Protein C4-300 4.6 mm ID x 5 cm L
0022828 TSKgel Protein C4-300 4.6 mm ID x 10 cm L
0022829 TSKgel Protein C4-300 4.6 mm ID x 15 cm L
0022833 TSKgel Guard Cartridge for 4.6. mm ID,
3 pieces/pkg
3.2 mm ID x 1.5 cm L
0019018 TSKgel Guard Cartridge holder 3.2 mm ID x 1.5 cm L