TSKgel TMS-250
TSKgel TMS-250 columns contain a unique C1 (TMS) bonded phase. The packing is prepared by monomeric binding of trimethyl silyl groups to a 25 nm (250 Å) pore size spherical silica. Due to the low hydrophobicity of the ligand, excellent recoveries are common even when used with large proteins. Proteins such as adolase (158 kDa) exhibit sharp peaks relative to wide pore C18 columns.
Structure of TSKgel TMS-250 |
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High resolution protein separation |
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Column: TSKgel TMS-250, 4.6 mm ID x 7.5 cm
Mobile phase: 60 min linear gradient from 20% to 95% CH3CN in 0.05% TFA, pH 2.2
Flow rate: 0.61 mL/min
Detection: UV @ 220 nm
Sample: 5 µg each of:
1 ribonuclease A,2. cytochrome C, 3. lysozyme,
4. bovine serum albumin, 5. aldolase,
6. carbonic anhydrase, 7. ovalbumin |