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TSKgel PWXL Columns

TSKgel PWXL Columns for Analysis of Water-Soluble Polymers

TSKgel PWXL columns are composed of spherical, hydrophilic polymethacrylate beads. The main application area for these columns is the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides. See the table on the TSKgel PW columns page for typical samples and suitable eluents.

The figures below show the calibration curves for all TSKgel PWXL columns. 

Calibration Curves of TSKgel PWXL

PWXL_Cal-curves1a.png

PWXL_Cal-curves2.png
Columns:




Mobile phase:
Flow rate:
Detection:
TSKgel PWXL columns,
all 7.8 mm ID × 30 cm
A. G2500PWXL, B. G3000PWXL
C. G4000PWXL, D. G5000PWXL,
E. G6000PWXL, F. GMPWXL
distilled H20
1.0 mL/min
RI
Columns:


Mobile phase:
Flow rate:
Detection:

Samples:
TSKgel PWXL columns, all 7.8 mm ID × 30 cm
1. G3000PWXL,  2. G4000PWXL,
3. G5000PWXL, 4. G6000PWXL, 5. GMPWXL
0.2 mol/L phosphate buffer, pH 6.8
1.0 mL/min
UV @280 nm
25 °C
a. thyroglobulin (6.6 × 105 Da)
b. g-globulin (1.5 × 105 Da)
c. albumin (6.7 × 104 Da)
d. ovalbumin (4.3 × 104 Da)
e. b-lactoglobulin (3.6 × 104 Da)
f. myoglobin (1.69 × 104 Da)
g. cytochrome C (1.24 × 104 Da)

G-DNA-PW_Cal-curves_a.png
G-Oligo_PW_Cal-curves_b.png  

SuperOligoPW_Cal-curves_b.png
Column:



                  Mobile phase:


Flow rate:
Detection:
Sample:
TSKgel G-DNA-PW,
10 µm,
7.8 mm ID × 30 cm × 4  
0.2 mol/L  H2O  with
0.3 mol/L NaCl in
0.1 mol/LTris-HCl,
pH 7.5, + 1 mmol/L EDTA
0.15 mL/min
UV @ 260 nm
Eco RI and Bst NI-cleaved pBR322 DNA, void volume determined with λ-DNA

Column:


Mobile phase:
Flow rate:
Detection:
Sample:
TSKgel G-Oligo-PW,
7 µm,
7.8 mm ID × 30 cm × 2
distilled H2O
1.0 mL/min
UV @ 260 nm
hydrolyzed
β-cyclodextrin
Column:

Mobile phase:
Flow rate:
Detection:
Temperature:
Samples:
TSKgel SuperOligoPW, 
6.0 mm ID × 15 cm
H2O
0.60 mL/min
RI
25 °C
PEO, PEG and ethylene glycol

Specialty TSKgel PWXL Columns

TSKgel G-DNA-PW is available for the analysis of large polynucleotides, such as DNA and RNA fragments of 500-5,000 base pairs.

TSKgel G-Oligo-PW is the preferred column for the high resolution analysis of aqueous nonionic and cationic oligmers and oligosaccharides such as hydrolyzed cyclodextrins. 

TSKgel SuperOligoPW contains smaller particles and semi-micro dimensions compared to the TSKgel G-Oligo-PW column, enabling high speed separations with high resolution of aqueous oligomers.

The mixed-mode column, TSKgel GMPWXL, has an extended linear calibration range, suitable for samples with a broad molar mass (MM) distribution and unknown samples.



Base Material:
hydroxylated polymethacrylate
Particle Size (mean): 7 µm
Pore Size (mean): < 20 nm
Calibration Range (polyethylene glycols and oxides): < 3,000 Da
Max % Organic: 20%
Shipping Solvent water

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Base Material:
hydroxylated polymethacrylate
Particle Size (mean):
7 µm
Pore Size (mean):
20 nm
Calibration Range (polyethylene glycols and oxides):
< 40,000 Da
Max % Organic:
20%
Shipping Solvent water

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Base Material: hydroxylated polymethacrylate
Particle Size (mean): 10 µm
Pore Size (mean): < 50 nm
Calibration Range (polyethylene glycols and oxides): 2,000 - 300,000 Da
Max % Organic: 20%
Shipping Solvent water

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Base Material: hydroxylated polymethacrylate
Particle Size (mean): 10 µm
Pore Size (mean): 100 nm
Calibration Range (polyethylene glycols and oxides): 4,000 - 800,000 Da
Max % Organic: 20%
Shipping Solvent water

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Base Material: hydroxylated polymethacrylate
Particle Size (mean): 13 µm
Pore Size (mean): > 100 nm
Calibration Range (polyethylene glycols and oxides): 40,000 - 8,000,000 Da
Max % Organic: 20%
Shipping Solvent water

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Base Material: hydroxylated polymethacrylate
Particle Size (mean): 10 µm
Pore Size (mean): > 100 nm
Working Range:  500 - 5,000 bp DNA 
Calibration Range (polyethylene glycols and oxides): 40,000 - 8,000,000 Da
Max % Organic: 20%
Shipping Solvent water

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Base Material: hydroxylated polymethacrylate
Particle Size (mean): 13 µm
Pore Size (mean): 10 - 100 nm
Calibration Range (polyethylene glycols and oxides): 1,000 - 8,000,000 Da
Max % Organic: 20%
Shipping Solvent water

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Base Material: hydroxylated polymethacrylate
Particle Size (mean): 7 µm
Pore Size (mean): 12.5 nm
Calibration Range (polyethylene glycols and oxides): up to 3,000 Da
Max % Organic: 20%
Shipping Solvent water

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Base Material: polymethacrylate
Particle Size (mean): 3 µm
Pore Size (mean): 12.5 nm
Calibration Range (polyethylene glycols and oxides): 100 - 3,000 Da
Max % Organic: 20%
Shipping Solvent water

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