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TSKgel SuperSW Columns

TSKgel SuperSW Columns for Higher Sensitivity Protein Analysis


TSKgel SuperSW columns, introduced in 1997, have become synonymous with analyzing protein molar masses in the emerging field of biotechnology.

TSKgel SuperSW columns contain smaller particles than TSKgel SWXL columns; 4 micron versus 5 micron. In addition, the column internal diameter has been reduced from 7.8 mm ID to 4.6 mm ID to provide higher sensitivity in sample-limited cases and to cut down on solvent use.

HPLC System Requirements

It is important to employ a UHPLC system or an HPLC system that is optimized with regards to extra-column bandbroadening to take full advantage of the high column efficiency that can be obtained on TSKgel SuperSW columns. See SEC Columns Brochure for recommendations.

Calibration Curves

The figures show the calibration curves for globular proteins (A) and for polyethylene oxides and glycols (B) for TSKgel SuperSW columns.

Calibration Curves of TSKgel SuperSW Columns

SuperSW Calibration Curves
 
Columns:        TSKgel SuperSW columns, 4.6 mm ID × 30 cm
Mobile phase: 0.2 mol/L phosphate buffer, pH 6.7
Flow rate:        0.35 mL/min
Detection:       UV @ 280 nm
Samples:         standard proteins (5 µL, 0.1 g/L each)
                        1. thyroglobulin, 2. gamma-globulin
                        3. bovine serum albumin
                        4. β-lactoglobulin
                        5. lysozyme, 6. cytochrome C
                        7. glycine tetramer 

 

Calibration Curves

 SuperSW_Cal-Curve2.png

Columns:        TSKgel SuperSW columns,4.6 mm ID × 30 cm
Mobile phase: 0.05% sodium azide aqueous solution
Flow rate:        0.35 mL/min
Detection:        RI
Temperature:  25 °C
Samples:         polyethylene oxides (PEO) standards polyethylene glycols (PEG)
                        standards, (5 µL) 

Tables within the tabs below summarize the molar mass ranges for the TSKgel SuperSW2000 and SuperSW3000 columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the particle pores.


Base Material:
Silica
Particle Size (mean):
µm
Pore Size (mean):
12.5 nm
Functional Group:
Diol
pH Stability:
2.5 - 7.5
Calibration Range (globular proteins):
5,000 - 150,000 Da
Calibration Range (dextrans):
1,000 - 3 x 10^4 Da
Calibration Range (polyethylene glycols and oxides):
500 - 15,000 Da

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Base Material:
Silica
Particle Size (mean):
µm
Pore Size (mean):
25 nm
Functional Group:
Diol
pH Stability:
2.5 - 7.5
Calibration Range (globular proteins):
1 x 10^4 - 5 x 10^5 Da
Calibration Range (dextrans):
2,000 - 7 x 10^4 Da
Calibration Range (polyethylene glycols and oxides):
1,000 - 3.5 x 10^4 Da

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