SOLUTIONS

Organic Size Exclusion - GPC


TSKgel Gel Permeation Chromatography Columns

Gel Permeation Chromatography (GPC) plays an important role in the characterization of polar organic-soluble and organic-soluble polymers in consumer, chemical and petrochemical industries.

TSKgel Alpha and TSKgel SuperAW columns are designed for the GPC analysis of polymers of intermediate polarity. These columns contain highly crosslinked hydroxylated polymethacrylate particles which minimize swelling in polar organic solvents.

TSKgel H series GPC columns are optimized for the analysis of organic-soluble polymers and are packed with porous spherical polystyrene divinylbenzene (PS-DVB) particles. This series includes TSKgel HXL, HHR, SuperH, SuperHZ, and SuperMultiporeHZ columns. Each line of columns within this series differs in degree of inertness, solvent compatibility and operating temperature range.

 

The TSKgel Alpha and SuperAW columns offer excellent stability in 100% aqueous to 100% organic solvents. Both column types are highly applicable for polymers solubilized in methanol, acetonitrile, DMSO, isopropanol, THF and HFIP. Examples of applications include cleansing gel in methanol, degree of saponification of polyvinylalcohol in HFIP, and sodium dodecylsulfate, polyimide, as well as cellulose derivatives in DMF with 10 mmol/L LiBr.

A mixed bed column is available in each of the TSKgel Alpha and SuperAW column line, which is prepared by mixing particles of different pore sizes, resulting in columns with an extended linear calibration curve. These columns are useful to quickly categorize the molar mass (MM) profile of an unknown sample and are used when a sample has a very broad MM distribution.

For high throughput applications, the smaller dimensions of the TSKgel SuperAW columns provide equivalent resolution in half the time and with minimal solvent waste compared to the TSKgel Alpha columns.

TSKgel Column Type Particle Size (µm)  Benefit 
Alpha » 7, 10, 13 Stable in a wide variety of 100% aqueous and 100% organic solvents; mixed bed column available for scouting purposes
SuperAW » 4, 6, 7, 9 Smaller dimensions allow for high efficiency, high speed analysis; mixed bed column available for scouting purposes

The TSKgel HXL columns are conventional GPC columns of 7.8 mm ID x 30 cm. These columns show true size exclusion behavior for most polymers, have limited solvent compatibility and maximum operating temperatures of 60 and 80 °C. Included in the TSKgel HXL column line are three mixed bed columns and a multi-pore column. Mixed bed columns contain particles with different pore sizes, providing an extended linear calibration curve. The multi-pore column provides a linear calibration curve due to each particle containing a range of pore sizes. One of the mixed bed columns is available for high temperature analysis up to 140 °C.

TSKgel SuperHZ columns are designed for high throughput/high efficiency GPC applications. These columns are based on the TSKgel HXL column line, differing only in dimensions (4.6 and 6.0 mm ID x 15 cm) and particle size.

TSKgel HHR columns are available in 7.8 mm ID x 30 cm and are compatible with a broad range of solvents. As with the TSKgel HXL columns, the TSKgel HHR column line consists of four mixed bed columns with maximum operating temperatures of 80 °C. The remaining columns within the TSKgel HHR line can be used up to 140 °C. In addition, nine TSKgel HHR columns are available for high temperature and ultra-high temperature analysis, with maximum operating temperatures of 140 °C and 220 °C, respectively.

TSKgel SuperH columns are high efficiency/high throughput versions of the conventional TSKgel HHR columns. These columns are 6.0 mm ID x 15 cm with maximum operating temperatures of 140 °C and can be used in a broad range of solvents.

The TSKgel HT columns are for high temperature applications (<140ºC), while the TSKgel HT2 columns are used in ultra-high temperature (up to 220ºC) applications.

Tosoh's innovative multi-pore technology, used in TSKgel SuperMultiporeHZ columns, produces particles that contain a broad range of pore sizes. The semi-micro dimensions of these columns, 4.6 mm ID x 15 cm, offer high speed analysis and low solvent consumption with precise results.

 

TSKgel Column Type  Particle Size (µm) Benefit
HXL »
5, 9 Conventional polymer analysis with ultra-low polymer adsorption; limited solvent compatibility range
SuperHZ »
3, 10 High throughput polymer analysis with ultra-low polymer adsorption; limited solvent compatibility range
HHR » 5, 13, 20, 30 Conventional polymer analysis with expanded solvent compatibility range
SuperH »
3, 5 High throughput polymer analysis with expanded
solvent compatibility range
HT Series » 13, 20, 30  High temperature analysis of polymers up to 140 °C 
HT2 Series » 20, 30 Ultra high temperature analysis of polymers
up to 220 °C  
SuperMultiporeHZ »
3, 4, 6 Ultra-high performance with low dead volume and wide pore distribution in each particle for superior linearity