SOLUTIONS

TSKgel Alpha Columns

TSKgel Alpha Columns for the Analysis of Polar Organic-Soluble Polymers

The main application area for TSKgel Alpha columns is the analysis of polymers that are soluble in polar organic solvents. Examples of applications include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in DMF with 10 mmol/L LiBr), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in HFIP. These columns contain highly crosslinked hydroxylated polymethacrylate particles which minimize swelling in polar organic solvents.

Mixed bed TSKgel Alpha Column

The mixed bed column, TSKgel Alpha-M, has an extended linear calibration range, and is suitable for samples with a broad molar mass (MM) distribution as well as samples with unknown molecular mass.

Calibration curves of all Alpha columns 

alpha_calibrationcurves.png
 
Column:          TSKgel Alpha columns, 7.8 mm ID × 30 cm
Mobile phase: A. H2O
                         B. MeOH containing 10 mmol/L LiBr
                         C. DMF containing 10 mmol/L LiBr
Flow rate:       1.0 mL/min
Detection:       RI
Temperature: A. 25 °C  B. 25 °C  C. 40 °C
Samples:         A, B: polyethylene glycol (PEG) and polyethylene oxide (PEO) standards
                         C. polystyrene (PS) standards

 

Unlike TSKgel PW-type columns, which are stable at most to a 50% organic mixed with water, TSKgel Alpha columns offer excellent stability in 100% aqueous to 100% organic solvents. Suitable organic solvents are shown in the figure below.


Alpha-3000_Solvent-compatibility.png 
Conditions of solvent change
Flow rate:          1.0 mL/min
Temperature:    25°C
Time for purge: 8 h


Conditions for TP measurement

Flow rate:         1.0 mL/min
Detection:          RI
Temperature:    25°C
Sample:             ethylene glycol

Base Material:
hydrophilic methacrylate
Particle Size (mean):
7 µm
Pore Size (mean):
2.5 nm
Exclusion Limit (Polyethylene oxide):
5,000 Da (water)
Exclusion Limit (Polystyrene THF):
10,000 Da
Shipping Solvent:
water
Max. Pressure Drop:
4 MPa

 

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Base Material:
hydrophilic methacrylate
Particle Size (mean):
7 µm
Pore Size (mean):
15 nm
Exclusion Limit (Polyethylene oxide):
90,000 Da (water)
Exclusion Limit (Polystyrene THF):
100,000 Da
Shipping Solvent:
water
Max. Pressure Drop:
4 MPa

 

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Base Material:
hydrophilic methacrylate
Particle Size (mean):
10 µm
Pore Size (mean):
45 nm
Exclusion Limit (Polyethylene oxide):
40,000 Da (water)
Exclusion Limit (Polystyrene THF):
1 Million Da
Shipping Solvent:
water
Max. Pressure Drop:
3 MPa

 

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Base Material:
hydrophilic methacrylate
Particle Size (mean):
10 µm
Pore Size (mean):
100 nm
Exclusion Limit (Polyethylene oxide):
1 Million Da (water)
Exclusion Limit (Polystyrene THF):
7 Million Da
Shipping Solvent:
water
Max. Pressure Drop:
3 MPa

 

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Base Material:
hydrophilic methacrylate
Particle Size (mean):
13 µm
Pore Size (mean):
ND
Exclusion Limit (Polyethylene oxide):
> 1 x 10^7 Da (water)
Exclusion Limit (Polystyrene THF):
> 10 Million Da
Shipping Solvent:
water
Max. Pressure Drop:
2 MPa

 

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Base Material:
hydrophilic methacrylate
Particle Size (mean):
13 µm
Exclusion Limit (Polyethylene oxide):
> 10^7 Da (water)
Exclusion Limit (Polystyrene THF):
> 10 Million Da
Shipping Solvent:
water
Max. Pressure Drop:
2 MPa

 

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