SOLUTIONS

TSKgel HHR Columns

TSKgel HHR Columns for Expanded Solvent Capability

The TSKgel HHR column line consists of eight columns with different pore sizes, TSKgel G1000HHR through TSKgel G7000HHR, and five columns with an extended linear range of the calibration curve. The calibration curves are shown below.

Main characteristics 
Maximum operating temperatures for TSKgel HHR columns is 140 °C for the conventional bed columns and 80 °C for the mixed bed columns. In addition, nine TSKgel HHR columns are available for high temperature and ultra-high temperature analysis, with maximum operating temperatures of 140 °C and 220 °C, respectively. 
Broad solvent range. All TSKgel HHR columns are shipped in THF, which can be exchanged for a wide variety of organic solvents as shown below. 
TSKgel HHR columns are conventional GPC columns with dimensions of 7.8 mm ID x 30 cm. Most HPLC systems do not require optimization of extra-column band broadening effects when working with conventional GPC columns. 


Columns with linear calibration curves

The TSKgel HHR product line contains five mixed bed columns, in which particles with different pore sizes are blended to provide an extended linear calibration curve. The mixd bed columns feature increasing linear calibration ranges, from TSKgel GMHHR-L, GMHHR-N, GMHHR-M, to GMHHR-H.

Calibration Curves on TSKgel HHR Columns 

fig1_hhr_calibration_curves.pngfig2_hhr_calibration_curves.png 
Column:          TSKgel HHR series, 7.8 mm ID x 30 cm
Sample:           polystyrene standards
Elution:           THF
Flow rate:       1.0 mL/min
Temperature: 25°C
Detection:       UV@ 254 nm 

 

Shipping Solvent Can Be Replaced With
Tetrahydrofuran 2,3 acetone, ethanol, quinoline, benzene, o-dichlorobenzene, ethyl acetate, dodecane, FC-113, carbon tetrachloride, dichloromethane, dichloroethane, trichloroethane, n-hexane, cyclohexane, xylene, tetrahydrofuran, chloroform, 1,4-dioxane, hexafluoroisopropanol, toluene, 1-chloronaphthalene, dimethylaceto acetamide, methyl ethyl ketone, trichlorobenzene, m-cresol, dimethylformamide, methylpyrrolidone, o-chlorophenol/chloroform, dimethyl sulfoxide, pyridine

Notes:

  • See table below for appropriate flow rates for solvent exchange. After switching to a very polar solvent, switching to a nonpolar is not recommended.
  • All TSKgel HHR analytical columns are shipped containing tetrahydrofuran (THF), except high temperature (HT) columns, which contain o-dichlorobenzene.
  • Prolonged exposure to carbon tetrachloride can corrode the stainless steel parts of a column and an HPLC system.

* 100% methanol cannot be used with TSKgel H-type columns; use this solvent with TSKgel SW-type or Alpha columns.

Solvent Recommended Flow Rate
n-Hexane
0.9
methyl ethyl ketone  0.7 
dichloromethane, ethyl acetate  0.6 
toluene, chloroform  0.5 
dimethylformamide 0.4 
carbon tetrachloride, pyridine  0.3 
dimethyl sulfoxide, dioxane, ethanol, N-methylpyrrolidone, o-dichlorobenzene  0.2 
quinoline, hexafluoroisopropanal, 1-chloronaphthalene  0.1 

 

Recommended Solvent Application
THF
polystyrene, epoxy resin, phenoxy resin, polycarbonate, polyisoprene, polyvinyl acetate, polyvinyl chloride, monoglycerides, fatty acids, poly(butadiene), polybutadiene, poly(methyl methacrylate), poly(styrene-butadiene), poly(styrene-acrylonitrile)
N,N-Dimethylformamide (DMF) +/- 5mM LiBr polyvinyl chloride, polyvinyl fluoride, urea resins, polyurethane, polystyrene, polyester,
polyimido ether, polyimido ester, polyphenol (aqueous solution), polyacrylonitrile
o-Dichlorobenzene (ODCB)
polyethylene, polypropylene
Chloroform
polycarboxylic ether, acrylic resin, epoxy resin, polystyrene
m-Cresol/Chloroform
nylon, polyester, polyamide, poly(ethylene terephthalate)
Toluene
polybutadiene, polysiloxane



Matrix:
polystyrene-divinylbenzene
Particle Size (mean):
5 µm
Pore Size (mean):
1.5 nm
Exclusion Limit:
< 1,000 Da
Max. Temperaure (°C):
140
Shipping Solvent:
THF

More Information


Matrix:
polystyrene-divinylbenzene
Particle Size (mean):
5 µm
Pore Size (mean):
2 nm
Exclusion Limit:
< 10,000 Da
Max. Temperaure (°C):
140
Shipping Solvent:
THF

More Information


Matrix:
polystyrene-divinylbenzene
Particle Size (mean):
5 µm
Pore Size (mean):
3 nm
Exclusion Limit:
20,000 Da
Max. Temperaure (°C):
140
Shipping Solvent:
THF

More Information


Matrix:
polystyrene-divinylbenzene
Particle Size (mean):
5 µm
Pore Size (mean):
7.5 nm
Exclusion Limit:
< 60,000 Da
Max. Temperaure (°C):
140
Shipping Solvent:
THF

More Information


Matrix:
polystyrene-divinylbenzene
Particle Size (mean):
5 µm
Pore Size (mean):
20 nm
Exclusion Limit:
< 400,000 Da
Max. Temperaure (°C):
140
Shipping Solvent:
THF

More Information


Matrix:
polystyrene-divinylbenzene
Particle Size (mean):
5 µm
Pore Size (mean):
65 nm
Exclusion Limit:
< 4 x 10^6 Da
Max. Temperaure (°C):
140
Shipping Solvent:
THF

More Information


Matrix:
polystyrene-divinylbenzene
Particle Size (mean):
5 µm
Pore Size (mean):
ND
Exclusion Limit:
< 4 x 10^7 Da
Max. Temperaure (°C):
140
Shipping Solvent:
THF

More Information


Matrix:
polystyrene-divinylbenzene
Particle Size (mean):
5 µm
Pore Size (mean):
ND
Exclusion Limit:
4 x 10^8 Da
Max. Temperaure (°C):
140
Shipping Solvent:
THF

More Information


Matrix:
polystyrene-divinylbenzene
Particle Size (mean):
5 µm
Pore Size (mean):
ND
Exclusion Limit:
< 4 x 10^8 Da
Max. Temperaure (°C):
140
Shipping Solvent:
THF

More Information


Matrix:
polystyrene-divinylbenzene
Particle Size (mean):
5 µm
Pore Size (mean):
mixed pore size
Exclusion Limit:
4 x 10^6
Max. Temperaure (°C):
140
Shipping Solvent:
THF

More Information


Matrix:
polystyrene-divinylbenzene
Particle Size (mean):
5 µm
Pore Size (mean):
mixed pore size
Exclusion Limit:
4 x 10^6
Max. Temperaure (°C):
140
Shipping Solvent:
THF

More Information


Matrix:
polystyrene-divinylbenzene
Particle Size (mean):
5 µm
Pore Size (mean):
3 nm
Exclusion Limit:
20,000 Da
Max. Temperaure (°C):
140
Shipping Solvent:
THF

More Information