SOLUTIONS

TOYOPEARL DEAE-650

TOYOPEARL DEAE-650

TOYOPEARL DEAE-650 is a weak anion exchange resin for biomolecule purification. It is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with diethylaminoethyl (DEAE) weak anion exchange groups. TOYOPEARL DEAE-650 has high chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates.

TOYOPEARL DEAE-650 can be used for high throughput capture, intermediate purification, and polishing process steps. It is frequently used in plasma fractionation and for the purification of recombinant blood coagulation factors. 

TOYOPEARL DEAE-650 is available in three particle sizes: 100 µm (C-grade), 65 µm (M-grade), and 35 µm (S-grade). If greater resolution is needed, the smaller particle TSKgel DEAE-5PW resin may be used. As both the TOYOPEARL DEAE-650 and TSKgel DEAE-5PW resins use the same ligand, the selectivity will be unchanged.

 

Structure of TOYOPEARL DEAE-650

DEAE650_structure.jpg

Product attributes

Pore size (mean): 100 nm
Particle size (mean): 100 µm (C-grade)
  65 µm (M-grade)
  35 µm (S-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years (estimated)

pKa values for ion exchange groups

deae_fig1.png

 

Selectivity comparisons of anion exchange resins

DEAE650_fig1.png
Resins:              A. TOYOPEARL GigaCap Q-650S
                           B. Competitor Product
                           C. Competitor Product
                           D. TOYOPEARL SuperQ-650S
                           E. TSKgel SuperQ-5PW (30)
                           F. TSKgel SuperQ-5PW (20)
                           G. TOYOPEARL DEAE-650S
                           H. TSKgel DEAE-5PW (30)
                           I. TSKgel DEAE-5PW (20)
Column size:     7.5 mm ID × 7.5 cm 
Mobile phase:   Buffer A: 50 mmol/L Tris-HCl, pH 8.5 
                           Buffer B: buffer A + 1.0 mol/L NaCl
Gradient:           0-100% buffer B (120 min)
Flow rate:         136 cm/hr (1.0 mL/min) 
Detection:         UV @ 280 nm
Injection vol.:   100 µL
Samples:            transferrin, 2.9 g/L
                           ovalbumin, 6.5 g/L
                           trypsin inhibitor, 10.0 g/L

 

Separation of human serum albumin fractions 

DEAE650_fig2.png

Resins:             1. TOYOPEARL DEAE-650S
                          2. TOYOPEARL DEAE-650MColumn size: 16 mm ID × 15 cm
Mobile phase:  Buffer A: 50 mmol/LTris-HCl, pH 8.6 Buffer B: 50 mmol/L Tris-HCl,
                          pH 8.6 + 0.5 mol/L NaCl
Gradient:          linear gradient from buffer A to buffer B in 200 mL total volume
Flow rate:         45 cm/hr (1.5 mL/min)
Detection:         UV @ 280 nm
Temperature:   25 ˚C
Sample:             human serum

Column:            TSKgel G3000SW, 7.5 mm ID × 30 cm × 2 in series
Mobile phase:   0.1 mol/L phosphate, pH 6.8 + 0.1 mol/L sodium sulfate
Detection:          UV @ 280 nm
Temperature:     25 ˚C
Sample:              1. crude human serum
                            2. albumin fraction from TOYOPEARL DEAE-650S
                            3. albumin fraction from TOYOPEARL DEAE-650M

 

Ordering Information

P/N Description Dimension
0007988 TOYOPEARL DEAE-650C 250 mL
0014694 TOYOPEARL DEAE-650C 1 L
0007989 TOYOPEARL DEAE-650C 5 L
0043201 TOYOPEARL DEAE-650M 100 mL
0007473 TOYOPEARL DEAE-650M 250 mL
0014693 TOYOPEARL DEAE-650M 1 L
0007974 TOYOPEARL DEAE-650M 5 L
0019804 TOYOPEARL DEAE-650S 25 mL
0007472 TOYOPEARL DEAE-650S 250 mL
0014692 TOYOPEARL DEAE-650S 1 L
0007973 TOYOPEARL DEAE-650S 5 L
0021360 ToyoScreen DEAE-650M 6 x 1 mL
0021361 ToyoScreen DEAE-650M 6 x 5 mL
0021392 ToyoScreen IEC Anion Mix Pack, (GigaCap Q-650M,
Q-600C AR, DEAE-650M, SuperQ-650M, QAE-550C)
5 x 1 mL
0021393 ToyoScreen IEC Anion Mix Pack, (GigaCap Q-650M,
Q-600C AR, DEAE-650M, SuperQ-650M, QAE-550C)
5 x 5 mL