TOYOPEARL GigaCap Q-650
TOYOPEARL GigaCap Q-650 is a high capacity, high resolution anion exchange resin optimized for the capture and purification of both small and large proteins, including oligonucleotides and monoclonal antibodies. It has excellent binding and elution kinetics, which results in a more concentrated protein fraction collected during the elution step.
TOYOPEARL GigaCap Q-650 is composed of polymethacrylate beads that have been chemically modified to provide a higher number of anionic binding sites and functionalized with quaternary amine (Q) strong anion exchange groups. It can be used for high throughput capture, intermediate purification, and polishing process steps. The resin is available in two particle sizes: 75 µm (M-grade) and 35 µm (S-grade).
TOYOPEARL GigaCap Q-650M exhibits typical dynamic binding capacities of about 175 g/L for BSA and 49 g/L for thyroglobulin, maintains high capacity at increased linear velocities and has good pressure-flow characteristics.
TOYOPEARL GigaCap Q-650S has dynamic binding capacities approaching 190 g/L for bovine serum albumin. This high capacity, combined with the 35 µm mean particle size, makes TOYOPEARL GigaCap Q-650S ideal for high resolution applications such as oligonucleotide purification.
Structure of TOYOPEARL GigaCap Q-650
|Pore size (mean): ||< 100 nm |
|Particle size (mean): ||75 µm (M-grade) |
35 µm (M-grade)
|Pressure rating: ||0.3 - 1.3 Mpa |
|Shipping buffer: ||20% ethanol |
|pH stability: ||3-13 |
|Shelf life: ||10 years (estimated) |
TOYOPEARL GigaCap Q-650M vs. Competitor Q elution pool volume comparison
|Column size: 6 mm ID x 40 mm L |
Sample: polyclonal human IgG (1 mg/mL)
Loading buffer: 15 mmol/LTris-HCl (pH= 8.7)
Elution buffer: 15 mmol/L Tris-HCl (pH= 8.7) + 1.0 mol/L NaCl
Linear velocity: 212 cm/h
Detection: UV @ 280 nm
Dynamic binding capacity of proteins with different molar masses @ 212 cm/hr
Oligonucleotide purification with TOYOPEARL GigaCap Q-650S
|Resin: TOYOPEARL GigaCap Q-650S |
Column size: 6.6 mm ID × 18.5 cm (6.3 mL)
Mobile phase: Buffer A: 20 mmol/L NaOH
Buffer B: 20 mmol/L NaOH, 3.0 mol/L NaCl
Gradient: 50% B (2 CV)
50-100% B (15 CV)
100% B (2 CV)
Flow rate: 200 cm/hr (1.14 mL/min)
Detection: UV @ 254 nm
Sample: crude phosphorothioate deoxyoligonucleotide
Sample load: 1.0 mg