SOLUTIONS

TOYOPEARL GigaCap Q-650

TOYOPEARL GigaCap Q-650

TOYOPEARL GigaCap Q-650 is a high capacity, high resolution anion exchange resin optimized for the capture and purification of both small and large proteins, including oligonucleotides and monoclonal antibodies. It has excellent binding and elution kinetics, which results in a more concentrated protein fraction collected during the elution step.

TOYOPEARL GigaCap Q-650 is composed of polymethacrylate beads that have been chemically modified to provide a higher number of anionic binding sites and functionalized with quaternary amine (Q) strong anion exchange groups. It can be used for high throughput capture, intermediate purification, and polishing process steps. The resin is available in two particle sizes: 75 µm (M-grade) and 35 µm (S-grade).

TOYOPEARL GigaCap Q-650M exhibits typical dynamic binding capacities of about 175 g/L for BSA and 49 g/L for thyroglobulin, maintains high capacity at increased linear velocities and has good pressure-flow characteristics.

TOYOPEARL GigaCap Q-650S has dynamic binding capacities approaching 190 g/L for bovine serum albumin. This high capacity, combined with the 35 µm mean particle size, makes TOYOPEARL GigaCap Q-650S ideal for high resolution applications such as oligonucleotide purification.

Structure of TOYOPEARL GigaCap Q-650

SuperQ650_structure.jpg

Product attributes

Pore size (mean): < 100 nm
Particle size (mean): 75 µm (M-grade)
35 µm (M-grade)
Pressure rating: 0.3 - 1.3 Mpa
Shipping buffer: 20% ethanol
pH stability: 3-13
Shelf life: 10 years (estimated)

TOYOPEARL GigaCap Q-650M vs. Competitor Q elution pool volume comparison

gigacapq_fig1.png
Column size:      6 mm ID x 40 mm L
Sample:              polyclonal human IgG (1 mg/mL)
Loading buffer: 15 mmol/LTris-HCl (pH= 8.7)
Elution buffer:   15 mmol/L Tris-HCl (pH= 8.7) + 1.0 mol/L NaCl
Linear velocity:  212 cm/h
Detection:           UV @ 280 nm


Dynamic binding capacity of proteins with different molar masses @ 212 cm/hr

gigacapq_fig2.png

Oligonucleotide purification with TOYOPEARL GigaCap Q-650S

gigacapq_fig3.png
Resin:              TOYOPEARL GigaCap Q-650S
Column size:   6.6 mm ID × 18.5 cm (6.3 mL)
Mobile phase: Buffer A: 20 mmol/L NaOH
                         Buffer B: 20 mmol/L NaOH, 3.0 mol/L NaCl
Gradient:         50% B (2 CV)
                         50-100% B (15 CV)
                         100% B (2 CV)
Flow rate:        200 cm/hr (1.14 mL/min)
Detection:       UV @ 254 nm
Sample:           crude phosphorothioate deoxyoligonucleotide
Sample load:  1.0 mg

 

Ordering Information

P/N Description Dimension
0022881 TOYOPEARL GigaCap Q-650S 25 mL
0022882 TOYOPEARL GigaCap Q-650S 250 mL
0022883 TOYOPEARL GigaCap Q-650S 1 L
0022884 TOYOPEARL GigaCap Q-650S 5 L
0022885 TOYOPEARL GigaCap Q-650S 50 L
0021854 TOYOPEARL GigaCap Q-650M 100 mL
0021855 TOYOPEARL GigaCap Q-650M 250 mL
0021856 TOYOPEARL GigaCap Q-650M 1 L
0021857 TOYOPEARL GigaCap Q-650M 5 L
0021859 ToyoScreen GigaCap Q-650M 6 x 1 mL
0021860 ToyoScreen GigaCap Q-650M 6 x 5 mL
0045104 MiniChrom TOYOPEARL GigaCap Q-650M, 5 mL 8 mm ID x 100 mm L
0045105 MiniChrom TOYOPEARL GigaCap Q-6500S, 5 mL 8 mm ID x 100 mm L
0045003 ToyoScreen RoboColumn GigaCap Q-650M 8 x 200 µL
0045004 ToyoScreen RoboColumn GigaCap Q-650M 8 x 600 µL
0021392 ToyoScreen IEC Anion Mix Pack, (GigaCap Q-650M,
Q-600C AR, DEAE-650M, SuperQ-650M, QAE-550C)
5 x 1 mL
0021393 ToyoScreen IEC Anion Mix Pack, (GigaCap Q-650M,
Q-600C AR, DEAE-650M, SuperQ-650M, QAE-550C)
5 x 5 mL
0021396 ToyoScreen IEC Mix Pack, (GigaCap CM-650M,
GigaCap S-650M, GigaCap Q-650M, Q-600C AR,
SuperQ-650M, SP-550C)
6 x 1 mL
0021397 ToyoScreen IEC Mix Pack, (GigaCap CM-650M,
GigaCap S-650M, GigaCap Q-650M, Q-600C AR,
SuperQ-650M, SP-550C)
6 x 5 mL