SOLUTIONS

TOYOPEARL NH2-750F

TOYOPEARL NH2-750F

TOYOPEARL NH2-750F is a salt tolerant anion exchange resin capable of aggregate removal in both flow-through and binding and elution modes. The resin is composed of polymethacrylate beads that have been functionalized with proprietary primary amine (NH2) strong anion exchange groups.

TOYOPEARL NH2-750F is ideal for process scale applications ranging from the capture of proteins from biological feedstock (mammalian cell culture, plasma, bacterial feedstock, etc.) without dilution to the intermediate or final purification of monoclonal antibodies (mAbs) where aggregates and other impurities are removed from the target of interest.

TOYOPEARL NH2-750F is available in 45 µm particle size (F-grade) and exhibits typical binding capacities of 70 g/L for BSA. It maintains its high capacity at increased linear velocities and has good pressure-flow characteristics.

 

Structure of TOYOPEARL NH2-750F   

NH2750_Structure.jpg 

 

Product attributes

Pore size (mean):  > 100 nm
Particle size (mean): 45 µm (F-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years (estimated)

 

Comparative salt tolerance of anion exchange resins 

nh2_fig1.png 
Resins:            various
Column size:   5 cm ID × 5 cm
Mobile phase: A: 20 mmol/L Tris-HCl, pH 8.0 B: mobile phase A + 2.0 mol/L NaCl
Gradient:         0 - 100% B (120 min)
Flow rate:       300 cm/hr (1.0 mL/min)
Detection:       UV @ 280 nm
Temperature: ambient
Sample:           BSA (1.0 g/L)

 

Separation of aggregates from IgG1 monomer on TOYOPEARL NH2-750F 

nh2_fig2.png 
Resin:              TOYOPEARL NH2-750F
Column size:   5 cm ID × 5 cm
Mobile phase: A: 20 mmol/L Tris-HCl, pH 8.0 B: mobile phase A + 1.0 mol/L NaCl
Gradient:         0 - 100% B (60 min)
Flow rate:        300 cm/hr (1.0 mL/min)
Detection:        UV @ 280 nm
Temperature: ambient
Sample:           mAb (IgG1) 0.5 g/L 

 

Ordering Information

P/N Description Dimension
0023438 TOYOPEARL NH2-750F 100 mL
0023439 TOYOPEARL NH2-750F 250 mL
0023440 TOYOPEARL NH2-750F 1 L
0023441 TOYOPEARL NH2-750F 5 L
0023442 TOYOPEARL NH2-750F 50 L
0045021 RoboColumn NH2-750F 200 µL x 8 
0045022 RoboColumn NH2-750F 600 µL x 8
0045108 MiniChrom NH2-750F 1 x 5 mL
0023443 ToyoScreen NH2-750F 6 x 1 mL
0023444 ToyoScreen NH2-750F 6 x 5 mL