SOLUTIONS

TSKgel SuperQ-5PW

TSKgel SuperQ-5PW

TSKgel SuperQ-5PW is a strong anion exchange resin for biomolecule purification. It is composed of highly crosslinked polymethacrylate beads that have been functionalized with quaternary amine (Q) strong anion exchange groups.

TSKgel SuperQ-5PW has high chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates. TSKgel PW resins vary from TOYOPEARL resins by having a higher degree of crosslinking which is necessitated by the higher flow pressures needed when using smaller particles for chromatography.

TSKgel SuperQ-5PW resins are best used for high resolution process polishing steps and the capture of oligonucleotides from crude harvest. TSKgel SuperQ-5PW is available in two particle sizes: 30 µm and 20 µm. Because similarly functionalized TSKgel PW and TOYOPEARL resin types have the same backbone polymer chemistry, the selectivity for proteins and impurities remains the same.

Product attributes

Pore size (mean): < 100 nm
Particle size (mean): 30 µm
20 µm
Pressure rating: 2 Mpa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years (estimated)

pKa values for Ion Exchange groups

deae_fig1.png
 

 

TSKgel SuperQ-5PW (20) maintains resolution at high oligonucleotide load 

 superq-5pw_fig2.png

Column:    0.66 cm x 15 cm L (5.1 mL) (resin as noted in figure)
Flow rate: 1.43 mL/min (250 cm/h)
                   Buffer A: 20 mmol/L Tris-HCl + 10 mmol/L EDTA (pH= 9.0)
                   Buffer B: 20 mmol/L Tris-HCl + 10 mmol/L EDTA + 1.0 mol/L NaCl (pH= 9.0)
Sample loaded: DNA based oligonucleotides were loaded as followed: 1 mg/column panels A & B, 20 mg/column panels C & D 
Separation conditions: Column was washed with 5 CV 100 % buffer A followed by 11 mL injection. Column was then washed with 3 CV 100 % buffer A followed by 6 CV of linear gradient 35-53 buffer B. Finally, column was washed with 5 CV 100 % buffer B.  
Detection: UV @ 254 nm
Fractions: 0.5 mL fractions were taken from peaks of interest and analyzed on a TSKgel DNA-NPR column

 


TSKgel SuperQ-5PW (20), 1.0 mg load 

 superq_fig2.png
Resin:              TSKgel SuperQ-5PW (20) 
Column size:   6.6 mm ID × 18.5 cm (6.3 mL)
Mobile phase: Buffer A: 20 mmol/L NaOH
                         Buffer B: 20 mmol/L NaOH, 3.0 mol/L NaCl
Gradient:        50% B (2 CV) 50-100% B (15 CV) 100% B (2 CV) 
Flow rate:       200 cm/hr (1.14 mL/min)
Detection:       UV @ 254 nmSample: crude phosphorothioate deoxyoligonucleotide
Sample load:  1.0 mg 
 

 

Ordering Information

P/N Description Dimension
0043283 TSKgel SuperQ-5PW (30) 25 mL
0018536 TSKgel SuperQ-5PW (30) 250 mL
0018548 TSKgel SuperQ-5PW (30) 1 L
0018549 TSKgel SuperQ-5PW (30) 5 L
0043383 TSKgel SuperQ-5PW (20) 25 mL
0018535 TSKgel SuperQ-5PW (20) 250 mL
0018546 TSKgel SuperQ-5PW (20) 1 L
0018547 TSKgel SuperQ-5PW (20) 5 L