SOLUTIONS

TOYOPEARL GigaCap S-650

TOYOPEARL GigaCap S-650

TOYOPEARL GigaCap S-650 media are high capacity, high resolution cation exchange resins optimized for the capture and purification of both small and large proteins, including monoclonal antibodies. They are composed of polymethacrylate beads that have been chemically modified to provide a higher number of cationic binding sites and functionalized with sulfopropyl (S) strong cation exchange groups.

TOYOPEARL GigaCap S-650 resins can be used for high throughput capture, intermediate purification, and polishing process steps. They are available in two particle sizes: 75 µm (M-grade), and 35 µm (S-grade). TOYOPEARL GigaCap S-650 media also have excellent binding and elution kinetics, which results in a more concentrated protein fraction collected during the elution step.

TOYOPEARL GigaCap S-650M exhibits typical dynamic binding capacities greater than 150 g/L for human IgG and maintains its high capacity at increased linear velocities.
TOYOPEARL GigaCap S-650S has a dynamic binding capacity greater than 160 g/L for human IgG. This high capacity, combined with the 35 µm mean particle size, makes TOYOPEARL GigaCap S-650S ideal for high resolution applications.

 

Structure of TOYOPEARL GigaCap S-650M

GigaCapS_structure.jpg 

 

Product attributes

Pore size (mean): < 100 nm
Particle size (mean): 100 µm (C-grade)
  65 µm (M-grade)
  35 µm (S-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years (estimated)

TOYOPEARL GigaCap S-650M vs. Competitor S elution pool volume comparison 

 Gigacaps competitor comparison
Column size:        6 mm ID x 40 mm bed
Sample:                polyclonal human IgG (1 mg/mL)
Loading Buffer:   0.1 mol/L acetate buffer (pH= 4.7)
Elution Buffer:     0.1 mol/L acetate buffer (pH= 4.7) + 1.0 mol/L NaCl
Linear velocity:   212 cm/h
Detection:            UV @ 280 nm 

 

TOYOPEARL GigaCap S-650M human IgG breakthrough curves at various linear velocities 

gigacaps_fig1.png 
Resins:            TOYOPEARL GigaCap S-650M
Column size:   6 mm ID × 4 cm (1.13 mL) 
Mobile phase: 0.1 mol/L acetate buffer, pH 4.7
Flow rates:      212, 424, 636, 1060 cm/hr (1.0, 2.0, 3.0, 5.0 mL/min)
Detection:       UV @ 280 nm
Sample:           polyclonal human IgG (1 g/L) 

 

Selectivity comparison between native protein and mono-PEGylated protein on TOYOPEARL GigaCap resins 

gigacaps_fig2.png 
Resins:            TOYOPEARL GigaCap S-650M TOYOPEARL GigaCap CM-650M
Column size:   6 mm ID × 4 cm
Mobile phase: Buffer A: 50 mmol/L phosphate buffer, pH 7.0
                         Buffer B: 50 mmol/L phosphate buffer, pH 7.0 + 0.5 mol/L NaCl
Gradients:       10 minute 100% buffer A 10 minute 100% buffer A 60 minutes
                         0%B to 100%B 60 minutes 0%B to 50%B
Flow rate:        212 cm/hr (1 mL/min)
Samples:         native lysozyme, 5 g/L mono-PEGylated lysozyme, 5 g/L
                         (PEG MW= 5 kDa) 

 

Ordering Information

P/N Description Dimension
0022875 TOYOPEARL GigaCap S-650S 25 mL
0022876 TOYOPEARL GigaCap S-650S 250 mL
0022877 TOYOPEARL GigaCap S-650S 1 L
0022878 TOYOPEARL GigaCap S-650S 5 L
0022879 TOYOPEARL GigaCap S-650S 50 L
0021833 TOYOPEARL GigaCap S-650M 100 mL
0021834 TOYOPEARL GigaCap S-650M 250 mL
0021835 TOYOPEARL GigaCap S-650M 1 L
0021836 TOYOPEARL GigaCap S-650M 5 L
0045102 MiniChrom TOYOPEARL GigaCap S-650S, 5 mL 8 mm ID x 100 mm ID
0045101 MiniChrom TOYOPEARL GigaCap S-650M, 5 mL 8 mm ID x 100 mm ID
0045023 RoboColumn GigaCap S-650S 200 x 8 µL
0045024 RoboColumn GigaCap S-650S 600 x 8 µL
0045001 RoboColumn GigaCap S-650M 200 x 8 µL
0045002 RoboColumn GigaCap S-650M 600 x 8 µL
0021868 ToyoScreen GigaCap S-650M 6 x 1 mL
0021869 ToyoScreen GigaCap S-650M 6 x 5 mL
0021394  ToyoScreen IEC Cation Mix Pack, (GigaCap CM-650M,
GigaCap S-650M, CM-650M, SP-650M, SP-550M)
5 x 1 mL 
0021395  ToyoScreen IEC Cation Mix Pack, (GigaCap CM-650M,
GigaCap S-650M, CM-650M, SP-650M, SP-550M) 
5 x 5 mL 
0021396  ToyoScreen IEC Mix Pack, (GigaCap CM-650M,
GigaCap S-650M, GigaCap Q-650M, Q-600C AR,
SuperQ-650M, SP-550C) 
6 x 1 mL 
0021397  ToyoScreen IEC Mix Pack, (GigaCap CM-650M,
GigaCap S-650M, GigaCap Q-650M, Q-600C AR,
SuperQ-650M, SP-550C)  
6 x 5 mL