TOYOPEARL Sulfate-650F is a salt tolerant cation exchange resin capable of aggregate removal in bind-elute mode. The resin is composed of polymethacrylate beads that have been functionalized with a proprietary sulfate containing ligand.
TOYOPEARL Sulfate-650F is ideal for process scale applications ranging from the capture of proteins from biological feedstock (mammalian cell culture, plasma, bacterial feedstock, etc.) without dilution to the intermediate or final purification of monoclonal antibodies (mAbs) where aggregates and other impurities are removed from the target of interest. In addition to it’s ion exchange function it also provides Heparin-like affinity for certain targets. TOYOPEARL Sulfate-650F is available in 45 µm particle size (F-grade) and exhibits typical binding capacities of over 95 g/L for IgG. It maintains its high capacity at increased linear velocities and has good pressure-flow characteristics up to 600 cm/h liner velocity.
Effect of pH and conductivity on IgG DBC
|Column: TOYOPEARL Sulfate-650F 6.0 mm ID.x 4 cm L |
Sample: 1 g/L polyclonal hIgG (Kakatsuken) in x mol/L NaCl
+ 0.054 mol/L acetate (pH 4.2-5.5) or MES buffer (pH 6.0, 6.5)
Residence time: 4 min
Aggregate removal on various cation exchange media
|Column: Cation exchanger, 7.5 mm ID X 7.5 cm L |
Eluents: A: 0.054 mol/L acetate buffer (pH 5.5), B: 1.0 mol/L NaCl in A
Gradient: 58.5 min linear 0% -100% B (NaCl conc. + 0.0167 M/min)
Flow rate: 1.0 mL/min
Sample: 3 g/L monoclonal humanized IgG acid/heat treatment,
Injection vol.: 90 µL (Aggregate content ~1.9%)