SOLUTIONS

Protein L for Antibody Purification

TOYOPEARL® AF-rProtein L-650F

High capacity chromatography resin for the capture of antibodies and antibody fragments

The new Protein L affinity resin combines the rigid TOYOPEARL polymer matrix with an engineered recombinant Protein L ligand. TOYOPEARL AF-r Protein L-650F exhibits dynamic binding capacities (DBCs) of greater than 38 g/L for Fabs, almost twice the capacity of other commercial Protein L resins.

Protein L has a strong affinity for the variable region of an antibody’s kappa light chain. TOYOPEARL AF-r Protein L-650F is therefore suitable for the purification of a wide range of antibody related molecules which do not bind to Protein A. Examples are antibody fragments (Fabs), domain antibodies (Dabs) and single chain variable fragments (scFv) as well as IgM and IgA.

The recombinant ligand that is linked to the well proven methacrylic polymer backbone of TOYOPEARL media has been engineered to maintain capacity even after repeated exposure to mild alkaline solution. Its multipoint attachment to the TOYOPEARL matrix further enhances its chemical stability. Due to its rigid polymer matrix TOYOPEARL AF-rProtein L-650F also provides excellent pressure flow characteristics.

Affinity resins constitute a substantial cost in process scale purification processes. The high capacity of the new TOYOPEARL AF-rProtein L-650F resin and its robustness can help to increase product throughput, reduce operating costs, and increase manufacturing productivity.

Recombinant Protein L derived ligand 

 proteinL_ligand-structure.jpg
The B4 domain was code optimized and expressed as a tetramer in E. coli. 

 

Dynamic binding capacity of Protein L media for Fab

 
proteinL_DBC-Fab.jpg 
Column:    TOYOPEARL AF-rProtein L-650F/competitor resin
                   4.6 mm ID × 50 mm (0.83 mL)
Detection: UV@280 nm
Sample:     2 g/L human Fab in 0.1 mol/L Na-Phosphate (pH 6.5)
DBC measured at 10 % Breakthrough 

 

Product attributes

Pore size (mean): 100 nm
Particle size (mean): 45 µm (F-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life (room temperature): 1 year (ongoing)

 

Ordering Information

P/N Description Dimension
0023486 TOYOPEARL AF-rProtein L-650F 10 mL
0023487 TOYOPEARL AF-rProtein L-650F 25 mL
0023488 TOYOPEARL AF-rProtein L-650F 100 mL
0023489 TOYOPEARL AF-rProtein L-650F 1 L
0023490 TOYOPEARL AF-rProtein L-650F 5 L
0045065 RoboColumn AF-rProtein L-650F 200 µL x 8 
0045066 RoboColumn AF-rProtein L-650F 600 µL x 8
0045162 MiniChrom AF-rProtein L-650M, 5 mL 8 mm ID x 10 cm L
0023494 ToyoScreen AF-rProtein L-650F 1 mL x 5
0023495 ToyoScreen AF-rProtein L-650F 5 mL x 1
0023496 ToyoScreen AF-rProtein L-650F 5 mL x 5
0045509 Resin Seeker AF-rProtein L-650F 20 µL x 96