TOYOPEARL® AF-rProtein L-650F
High capacity chromatography resin for the capture of antibodies and antibody fragments
The new Protein L affinity resin combines the rigid TOYOPEARL polymer matrix with an engineered recombinant Protein L ligand. TOYOPEARL AF-r Protein L-650F exhibits dynamic binding capacities (DBCs) of greater than 38 g/L for Fabs, almost twice the capacity of other commercial Protein L resins.
Protein L has a strong affinity for the variable region of an antibody’s kappa light chain. TOYOPEARL AF-r Protein L-650F is therefore suitable for the purification of a wide range of antibody related molecules which do not bind to Protein A. Examples are antibody fragments (Fabs), domain antibodies (Dabs) and single chain variable fragments (scFv) as well as IgM and IgA.
The recombinant ligand that is linked to the well proven methacrylic polymer backbone of TOYOPEARL media has been engineered to maintain capacity even after repeated exposure to mild alkaline solution. Its multipoint attachment to the TOYOPEARL matrix further enhances its chemical stability. Due to its rigid polymer matrix TOYOPEARL AF-rProtein L-650F also provides excellent pressure flow characteristics.
Affinity resins constitute a substantial cost in process scale purification processes. The high capacity of the new TOYOPEARL AF-rProtein L-650F resin and its robustness can help to increase product throughput, reduce operating costs, and increase manufacturing productivity.
Recombinant Protein L derived ligand
|The B4 domain was code optimized and expressed as a tetramer in E. coli. |
Dynamic binding capacity of Protein L media for Fab
|Column: TOYOPEARL AF-rProtein L-650F/competitor resin |
4.6 mm ID × 50 mm (0.83 mL)
Detection: UV@280 nm
Sample: 2 g/L human Fab in 0.1 mol/L Na-Phosphate (pH 6.5)
DBC measured at 10 % Breakthrough