Process Media Process Steps
The purification and recovery of a biological target molecule out of a tissue, cell or fermentation broth - the so called downstream processing - usually requires a combination of separation technologies and involves more than one chromatographic unit operation.
Downstream processing operations are often divided into three groups describing the continuous improvements in purity and concentration of the product: Capture, Intermediate Purification and Polishing. In parallel to the dimensions of the chromatography columns applied also the particle sizes of the chromatographic resins decrease along the progress of the purification train.
Quality control methods applied to confirm the purity of the final product also involve liquid chromatography. A variety of TSKgel (U)HPLC columns are available for QC analysis of biologics.