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Antibodies

Antibodies

Within the field of biologics, the majority of R &D is currently focused on recombinant proteins and monoclonal antibodies (mAbs). The top three selling drugs are all mAbs. A total of over 40 antibody products and derivatives have been approved by EMA and FDA so far. Today, more than 400 antibodies, primarily involving immunological and oncological targets, are under pre-clinical development and clinical trials. Tosoh Bioscience offers a variety of (U)HPLC columns for mAb analysis and TOYOPEARL and TSKgel media for purification of antibody related products.

 

 

 

TSKgel columns are used in a broad range of typical analysis in R&D and production of monoclonal antibodies: 
Aggregate and fragment content by Gel Filtration Chromatography (GFC) »
Glycan mapping by Hydrophilic Interaction Liquid Chromatography (HILIC) » 
Charge variants through Cation Exchange Chromatography » 
Reversed Phase analysis of fragments » 
Drug-to-antibody ratio (DAR) of antibody drug conjugates through Hydrophobic Interaction Chromatography (HIC) » 




TOYOPEARL and TSKgel media can be applied at every stage of a typical antibody purification platform: 
MAb capturing through high capacity Protein A Affinity Chromatography »
Host cell protein reduction through Cation Exchange Chromatography » 
DNA and virus removal through Anion Exchange Chromatography »  
Aggregate removal through Mixed-Mode Chromatography »  
Aggregate removal through salt tolerant Anion Exchange Chromatography » 
Polishing steps using Hydrophobic Interaction Chromatography »