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APPENDIX
APPENDIX
APPENDIX C
United States Pharmacopeia (USP) specifications and corresponding
Tosoh Bioscience columns
L1 - Octadecyl silane chemically bonded to porous silica or ceramic
micro-particles, 1.5 to 10 µm in diameter, or a monolithic rod.
Recommendations: TSKgel ODS-100V, ODS-100Z, ODS-100S,
Super-ODS, ODS-80TM, ODS-80TS, ODS-120A, ODS-120T
See: Reversed Phase section
L7 - Octylsilane chemically bonded to totally porous silica particles, 1.5
to 10 µm in diameter.
Recommendations: TSKgel Super-Octyl, Octyl-80TS
See: Reversed Phase section
L8 - An essentially monomolecular layer of aminopropylsilane
chemically bonded to totally porous silica gel support, 1.5 to 10 µm
in diameter.
Recommendations: TSKgel NH
2
-100, TSKgel NH
2
-100 DC
See: Hydrophilic Interaction section
L-9 - Irregular or spherical, totally porous silica gel having a
chemically bonded, strongly acidic cation-exchange coating, 3 to
10 µm in diameter.
Recommendations: TSKgel SP-2SW
See: Ion Exchange section
L10 - Nitrile groups chemically bonded to porous silica particles, 3 to
10 µm in diameter.
Recommendations: TSKgel CN-80TS
See: Reversed Phase section
L11 - Phenyl groups chemically bonded to porous silica particles, 1.5
to 10 µm in diameter.
Recommendations: TSKgel Super-Phenyl
See: Reversed Phase section
L13 - Trimethylsilane chemically bonded to porous silica particles, 3 to
10 µm in diameter.
Recommendations: TSKgel TMS-250
See: Reversed Phase section
L14 - Silica gel having a chemically bonded, strongly basic quaternary
ammonium anion exchange coating, 5 to 10 µm in diameter.
Recommendations: TSKgel QAE-2SW
See: Ion Exchange section
L20 - Dihydroxypropane groups chemically bonded to porous silica
particles, 5 to 10 µm in diameter.
Recommendations: TSKgel QC-PAK 200 and 300, SW
XL
, SW,
SuperSW, and SW mAb series
See: Size Exclusion section
L21 - A rigid, spherical styrene-divinylbenzene copolymer, 5 to 10 µm
in diameter
Recommendations: TSKgel H
XL
and H
HR,
SuperH, SuperHZ, and
SuperMultipore HZ series
See: Size Exclusion section
L22 - A cation-exchange resin made of porous polystyrene gel with
sulfonic acid groups, about 10 µm in size
Recommendations: TSKgel SCX
See: Ion Exchange section
L23 - An anion-exchange resin made of porous polymethacrylate or
polymethacrylate gel with quartenary ammonium groups, about 10
µm in size.
Recommendations: TSKgel SuperQ-5PW, BioAssist Q, Q-STAT, and
DNA-STAT
See: Ion Exchange section
L24- A semi-rigid hydrophilic gel consisting of vinyl polymers with
numerous hydroxyl groups in the matrix surface, 32 to 63 µm in
diameter.
Recommendations: TOYOPEARL HW-type
See: Size Exclusion in the Bulk Resin section
L25- Packing having the capacity to separate compounds with
a molecular weight range from 100-5000 (as determined by
polyethylene oxide), applied to neutral, anionic, and cationic water
soluble polymers.
Recommendations: TSKgel G2500PW, G2500PW
XL
, Alpha-2500,
SuperAW2500
See: Size Exclusion section
L26- Butyl silane chemically bonded to totally porous silica, 1.5 to 10
µm in diameter.
Recommendations: TSKgel Protein C
4
-300
See: Reversed Phase section
L33- Packing having the capacity to separate dextrans by molecular
size over a range of 4,000 to 500,000 daltons. It is spherical, silica-
based, and processed to provide pH stability.
Recommendations: TSKgel SuperSW, SW
XL
, QC-PAK, SW, and
Super mAb series
See: Size Exclusion section
L37- Packing having the capacity to separate proteins by
molecular size over a range of 2,000 to 40,000 daltons. It is a
polymethacrylate gel.
Recommendations: TSKgel G3000PW
XL
, G3000PW, G3000PW
XL
-CP
See: Size Exclusion section
