Type of Polymer TSKgel PW Columns for Analysis of Water-Soluble Polymers
TSKgel PW columns are composed of spherical, hydrophilic polymethacrylate beads. Particle sizes range from 12 µm for the smaller pore size columns to 17 µm for the larger pore size columns.
The main application areas for TSKgel PW columns are the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides.
The figure below shows the calibration curves for all TSKgel PW columns. Suitable solvents for various polymer samples are shown in the table below.
Calibration Curves or TSKgel PW Columns
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Columns: TSKgel PW columns, all 7.5 mm ID × 60 cm
A. G2000PW, B. G2500PW, C. G3000PW, D. G4000PW,
E. G5000PW, F. G6000PW, G. GMPW
Mobile phase: distilled H20
Flow rate: 1.0 mL/min
Detection: RI |
Specialty TSKgel PW Columns
The TSKgel GMPW column is a mixed bed column containing a mixture of different pore sizes that has an extended linear calibration range, suitable for samples with a broad molar mass (MM) distribution, as well as unknown samples. A TSKgel G6000PW column is available in PEEK hardware, TSKgel BioAssist G6PW, when ultra-low sample adsorption is required, such as in virus analysis.
Recommended eluents for GFC of water-soluble polymers on TSKgel PW columns
| Type of Polymer |
Typical Sample |
Suitable Eluent |
| Nonionic hydrophilic |
polyyethylene glycol |
distilled water |
| Anionic Hydrophilic |
sodium chondroitin sulfate, sodium alginate, carboymethyl cellulose, sodium polyacrylate, sodium hyaluronate |
Buffer or salt solution (e.g. 0.1 mol/L NaNO3) |
| Anionic Hydrophobic |
sulfonated lignin sodium salt, sodium polystyrenesulfonate |
Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 mol/L NaNO3) |
| Cationic Hydrophilic |
glycol chitosan, DEAE-dextran, poly(ethyleneimine), poly(trimethylaminoethyl methacrylate) iodide salt |
0.5 mol/L acetic acid with 0.3 mol/L Na2SO4 or 0.8 mol/L NaNO3 |
| Cationic Hydrophobic |
poly(4-vinylbenzyltrimethylammonium chloride), poly(N-methyl-2-vinylpyridinium) iodide salt |
0.5 mol/L acetic acid with 0.3 mol/L Na2SO4 |
| Amphoteric Hydrophilic |
peptides, proteins, poly- and oligosaccharides, DNA, RNA |
Buffer or salt solution (e.g. 0.1 mol/L NaNO3) |
| Amphoteric hydrophobic |
blue dextran, collagen, gelatin, hydrophobic proteins, hydrophobic peptides |
Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 mol/L NaNO3 or 35-45% CH3CN in 0.1% TFA) |