TSKgel FcR-IIIA Columns

TSKgel FcR-IIIA-NPR  Affinity Column for fast Evaluation of ADCC Activity

TSKgel FcR-IIIA-NPR is based on a recombinant Fc gamma IIIa receptor ligand immobilized on a non-porous polymer particle. It allows fast assessment of biologic activity of monoclonal antibodies.

Fc gamma IIIa receptor plays a key role in antibody-dependent cell mediated cytotoxicity (ADCC) which is a crucial mechanism of action of anti-tumor mAbs. The Fc-glycans of antibodies are known to play an important role in Fc-mediated effector functions. Hence, separation patterns of therapeutic antibodies on TSKgel FcR-IIIA-NPR can be correlated to Fc N-glycans and the ADCC activity of immunoglobulins. Early eluting peaks of TSKgel FcR-IIIA-NPR represent glycoforms with low ADCC activity while late eluting peaks represent glycoforms with high ADCC activity.

The rapid thirty-minute separation allows the analysis of large numbers of mAb samples to gain valuable first information on the distribution of glycoforms and expected ADCC activity. This fast and efficient method can be applied to purified samples and supernatant alike and can therefore be used in many phases of development and production such as cell line screening in early R&D, biosimilar/originator comparison, upstream development and optimization, monitoring of glycoengineering, or lot-to-lot comparison in QC.

FcR-IIIA affinity chromatography separates mAb glycoforms according to their ADCC activity

FcR Chromatogram

Column durability - analysis of CHO supernatant 



Base matrix  hydrophilic non-porous polymer
Particle size 5 µm
pH stability 4 – 8 (short term); 5-7 (long term)
Ligand modified recombinant human FcγRIIIA

Base matrix  hydrophilic porous polymer
Particle size 10 µm
pH stability 4 – 8 (short term); 5-7 (long term)
Ligand modified recombinant human FcγRIIIA

Ordering Information

P/N Description Dimension
0023513 TSKgel FcR-IIIA-NPR 4.6 mm ID x 7.5 cm L