TOYOPEARL Butyl-600M is based on a 75 nm (750 Å) pore size polymethacrylate base material bonded with C4 groups. It has the second highest hydrophobicity of the TOYOPEARL HIC resins. Its pore size has been optimized for the purification of monoclonal antibodies and is between that of TOYOPEARLl Butyl-650 and TOYOPEARL SuperButyl-550 media.

TOYOPEARL Butyl-600M is typically used for intermediate purification or polishing steps.


Structure of TOYOPEARL Butyl-600 resins 



Product attributes

Pore size (mean): 75 nm
Particle size (mean): 65 µm (M-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: >1-13
Shelf life: 10 years (estimated)

Comparison of TOYOPEARL -600M resins

Resins:            A. TOYOPEARL PPG-600M
                         B. TOYOPEARL Phenyl-600M
                         C. TOYOPEARL Butyl-600M
Column size:  7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 1.8 mol/L (NH4)2SO4 + 0.1 mol/L sodium phosphate, pH 7.0
                         Buffer B: 0.1 mol/L sodium phosphate, pH 7.0
Gradient:        60 min linear gradient from buffer A to B
Flow rate:       136 cm/hr (1.0 mL/min)
Detection:       UV @ 280 nm
Temperature: ambient
Samples:        1 g/L of: 1. ribonuclease A, 2.lysozyme, 3. α-chymotrypsinogen
Load volume: 100 µL

Breakthrough curves of polyclonal IgG on various HIC resins


Resins:           A. TOYOPEARL PPG-600M
                        B. TOYOPEARL Phenyl-650M
                        C. TOYOPEARL Butyl-600M
                        D. Competitor Phenyl Agarose
                        E. TOYOPEARL Phenyl-600M
Column size:  7.8 mm ID × 20 cm
Mobile phase: 0.1 mol/L sodium phosphate, pH 7.0 + 0.8 mol/L (NH4)2SO4
Flow rate:       300 cm/hr (2.4 mL/min)
Detection:       UV @ 280 nm
Temperature: 25 °C
Samples:         1.0 g/L polyclonal IgG

DBC was calculated at 10% breakthrough


Ordering Information

P/N Description Dimension
0021448 TOYOPEARL Butyl-600M 25 mL
0021449 TOYOPEARL Butyl-600M 100 mL
0021450 TOYOPEARL Butyl-600M 1 L
0021451 TOYOPEARL Butyl-600M 5 L
0045236 SkillPak HIC-600 1 mL col. Library
(Butyl-600M, Phenyl-600M, PPG-600M 1 mL x 2 ea)
7 mm ID x 2.5 cm L
0045214 SkillPak Butyl-600M 5 x 1 mL col. 7 mm ID x 2.5 cm L
0045250 SkillPak Butyl-600M 5 mL col. 8 mm ID x 10 cm L