TOYOPEARL Hexyl-650 is based on a 100 nm (1000 Å) pore size polymethacrylate base material bonded with C6 groups. This resin has the highest hydrophobicity of the TOYOPEARL HIC resins. It is used for very hydrophilic proteins or where a low salt elution environment is required. It is useful as an early capture step or for intermediate purification.

TOYOPEARL Hexyl-650 is available in three particle sizes, S-, M-, and C-grade.


Structure of TOYOPEARL Hexyl-650 resins 



Product attributes

Pore size (mean): 100 nm
Particle size (mean): 100 µm (C-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: >1-13
Shelf life: 10 years (estimated)

Typical dynamic binding capacities for humanized mAb

                                                Binding capacitiy (mg/mL - gel)
                                                (10% breakthrough)
Phenyl-650M                                           35.2
Butyl-650M                                             42.1
PPG-600M                                              44.8
Butyl-600M                                             48.5 

Selectivity of TOYOPEARL HIC resins

Samples: 1. Ribonuclease A, 2. Lysozyme, 3. α- Chymotrypsinogen


Ordering Information

P/N Description Dimension
0044465 TOYOPEARL Hexyl-650C 25 mL
0019026 TOYOPEARL Hexyl-650C 100 mL
0019027 TOYOPEARL Hexyl-650C 1 L
0019028 TOYOPEARL Hexyl-650C 5 L
0045233 SkillPak HIC 1 mL col. Library
(Butyl-650M, Phenyl-650M, PPG-600M, Hexyl-650C,
Ether-650M 1 mL x 1 ea)
7 mm ID x 2.5 cm L
0045219 SkillPak Hexyl-650C 5 x 1 mL col. 7 mm ID x 2.5 cm L
0045255 SkillPak Hexyl-650C 5 mL col. 8 mm ID x 10 cm L