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TOYOPEARL Phenyl-650

TOYOPEARL Phenyl-650

TOYOPEARL Phenyl-650 is based on a 100 nm (1000 Å) pore size polymethacrylate base material bonded with phenyl groups. This resin has an intermediate hydrophobicity. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in intermediate purification and polishing steps. For high resolution separations, the use of TSKgel Phenyl-5PW media is suggested. Both products have the same selectivity.

TOYOPEARL Phenyl-650 is available in three particle sizes, S-, M-, and C-grade.

 

Structure of TOYOPEARL Phenyl-650 resins 

Phenyl650_structure.png
 

 

Product attributes

Pore size (mean): 100 nm
Particle size (mean): 100 µm (C-grade)
  65 µm (M-grade)
  35 µm (S-grade)
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: >1-13
Shelf life: 10 years (estimated)

Typical dynamic binding capacities for humanized mAb

HIC_fig2.png 
                                                Binding capacitiy (mg/mL - gel)
                                                (10% breakthrough)
Phenyl-650M                                           35.2
Butyl-650M                                             42.1
PPG-600M                                              44.8
Butyl-600M                                             48.5 

Selectivity of TOYOPEARL HIC resins

HIC_fig3.png
Samples: 1. Ribonuclease A, 2. Lysozyme, 3. α- Chymotrypsinogen

 

Ordering Information

P/N Description Dimension
0043152 TOYOPEARL Phenyl-650S 25 mL
0014477 TOYOPEARL Phenyl-650S 100 mL
0014784 TOYOPEARL Phenyl-650S 1 L
0014935 TOYOPEARL Phenyl-650S 5 L
0019818 TOYOPEARL Phenyl-650M 25 mL
0014478 TOYOPEARL Phenyl-650M 100 mL
0014783 TOYOPEARL Phenyl-650M 1 L
0014943 TOYOPEARL Phenyl-650M 5 L
0043126 TOYOPEARL Phenyl-650C 25 mL
0014479 TOYOPEARL Phenyl-650C 100 mL
0014785 TOYOPEARL Phenyl-650C 1 L
0014944 TOYOPEARL Phenyl-650C 5 L
0014944 TOYOPEARL Phenyl-650C 5 L
0045233 SkillPak HIC 1 mL col. Library
(Butyl-650M, Phenyl-650M, PPG-600M, Hexyl-650C,
Ether-650M 1 mL x 1 ea)
7 mm ID x 2.5 cm L
0045217 SkillPak Phenyl-650M 5 x 1 mL col. 7 mm ID x 2.5 cm L
0045253 SkillPak Phenyl-650M 5 mL col. 8 mm ID x 10 cm L