6
GPC
EcoSEC HIGH TEMPERATURE GPC SYSTEM
PERFORMANCE MEANS REPRODUCIBILITY
The dual flow design of the RI detector and the temperature
controlled pumps of the EcoSEC High Temperature GPC
System deliver precise flow rates at all temperatures, even
when changes in environmental conditions occur, thus
producing reproducible results sample after sample, day
after day. The intraday and day-to-day reproducibility of the
EcoSEC High Temperature GPC System are shown in
Figure 4.
The engineering design concepts of the EcoSEC High
Temperature GPC System result in a high degree of repro-
ducibility of retention times (Figure 5A) and molar mass
determinations (Figure 5B). The coefficients of variation
for retention time and weight-average molar mass, M
w
, are
well below 1% for successive injections.
2.0 × 10
5
1
Molar mass (g/mol)
Retention time (minutes)
Number of injections
2
3
4
5
6
7
8
9
10
0
A
B
2.1 × 10
5
2.2 × 10
5
2.3 × 10
5
2.4 × 10
5
2.5 × 10
5
15.0
15.2
15.4
15.6
15.8
16.0
1
Number of injections
2
3
4
5
6
7
8
9
10
0
CV = 0.068%
CV = 0.061%
A: INTRADAY RETENTION TIME REPRODUCIBILITY, B: INTRADAY
WEIGHT-AVERAGE MOLAR MASS REPRODUCIBILITY
Column: TSKgel GMH
HR
-H(S) HT2, 13 µm, 7.8 mm ID × 30 cm L × 2;
Mobile phase: ODCB with 0.05% BHT; Flow rate: 1.0 mL/min;
Detection: RI (EcoSEC High Temperature GPC System);
Temperature: 145 °C; Injection vol.: 300 µL; Sample: polypropylene
FIGURE 5
0
10
20
30
0
5
10
15
Column:
TSKgel GMH
HR
-H(S)HT2
Solvent:
ODCB, 1.0 mL/min, 145 ˚C
Flow Rate:
1.0 mL/min
Temperature:
145 ˚C
Sample:
Polystyrene (F-20), 0.02 %, 300 µL
Detector response (mV)
Retention time (minutes)
Injection #1
Injection #2
Injection #3
Injection #4
Injection #5
Injection #6
GPC ELUTION PROFILE OF INTRADAY REPRODUCIBILITY OF THE
EcoSEC HIGH TEMPERATURE GPC SYSTEM
Reproducibility (intraday, n=6)
R.T.: CV 0.017%; Area: CV 0.42%
Reproducibility (day to day, n=5)
R.T.: CV 0.047%; Area: CV 0.71%
Column: TSKgel GMH
HR
-H(S) HT2, 13 µm, 7.8 mm ID × 30 cm L × 2;
Mobile phase: ODCB with 0.05% BHT; Flow rate: 1.0 mL/min;
Detection: RI (EcoSEC High Temperature GPC System); Temp: 145 °C
Injection vol.: 300 µL; Sample: polystyrene (F-20), 0.02%
FIGURE 4
