Specialty RP Silica Columns
There are three specialty silica-based TSKgel reversed phase columns: TSKgel ODS-120A, TSKgel ODS-120T, and TSKgel OligoDNA-RP.
TSKgel ODS-120A columns use a 15 nm pore size silica base support. The bonding method results in a polymeric coverage of C18 groups on the silica surface. The "A" signifies that the material is not endcapped. The silica base support's exclusion limit of 1 × 104 Da makes them a good choice for the reversed phase chromatography of peptides, small proteins, and environmental samples such as poly-aromatic hydrocarbons.
TSKgel ODS-120T columns also use a 15 nm pore size silica base support. The columns are endcapped with trimethyl silane groups to improve the peak shape of negatively charged analytes.
Specifically designed for the purification of oligonucleotides, RNA and DNA fragments (up to 500 mer), TSKgel OligoDNA RP columns provide excellent separations of samples with very similar sequences. The packing is prepared by monomeric binding of octadecyl silyl groups to 5 µm spherical silica gel with 25 nm pores. This packing is not endcapped and it has a relatively low carbon content of 10%.