TSKgel ODS-120T
TSKgel ODS-120T columns are based on a 15 nm pore size silica base support bonded with ODS groups. The columns are endcapped with TMS to improve the peak shape of negatively charged analytes. TSKgel ODS-120T has an effective pore size of 12 nm (120 Å).
TSKgel ODS-120T structure |
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Purification and rapid analysis of synthetic peptides |
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Column: TSKgel ODS-120T, 5 µm, 4.6 mm ID × 15 cm
Mobile phase: 48 min linear gradient from 14% to 50% CH3CN in 0.1% TFA
Flow rate: 1.0 mL/min
Detection: UV @ 215 nm
Sample: triacontadipeptide
(EAEDLQVGQVELGGGPGAGSLQPLALEGSLQC)
indicated by arrow; 50 µg in 50 µL |