FAQs on Analysis with Antibody Affinity Chromatography

FAQs on Analysis with Antibody Affinity Chromatography

Which ligands does Tosoh offer to analyze antibodies by affinity chromatography?

We offer two ligands for HPLC affinity analysis of antibodies:

  • Protein A–based separation is used to quickly determine antibody titer in supernatants

Figure 1: Titer determination of a monoclonal antibody (mAb) with TSKgel Protein A–5PW

Titer determination of a monoclonal antibody (mAb) with TSKgel Protein A–5PW
  • Fc-receptor-based separation retains antibodies according their affinity to the specific Fc-receptor FcγRIIIA and is used to determine Fc-receptor affinity, to estimate glycan-pattern or antibody-dependent cellular cytotoxicity activity (ADCC).