RoboColumns

Related literature - ToyoScreen RoboColumns

Flyer - RoboColumns

RoboColumns Manual

Process Media - Find your best fit!

Purification of Biomolecules - Liquid Chromatography Modes

Bioprocessing Application Notebook

RoboColumns

RoboColumns are miniaturized chromatographic columns pre-packed with TOYOPEARL ion exchange, mixed-mode, hydrophobic interaction or affinity media. Available in different volumes, they are designed to operate with a robotic liquid handling system, such as the Freedom EVO® from TECAN. This approach allows automated high-throughput, small-scale bio-chromatographic separations of protein samples by running up to eight individual columns simultaneously. RoboColumns are packed with TOYOPEARL to our specifications by Repligen and are supplied in strips of 8 columns.

RoboColumns can be used in a wide range of applications, including individual and parallel resin screening, optimization of separation conditions, scale-down experiments, as well as high throughput sample preparation. Binding and elution conditions, washing parameters, etc. can be investigated to explore the design space of the particular molecule’s purification process.

Optimization of anion exchange conditions

Elution profile of a protein A capture eluate on RoboColumns packed with TOYOPEARL SuperQ-650M at various conditions. Data kindly provided by T. Schröder, Repligen GmbH.

RoboColumns for fast automated screening of resins

0045099

Array Plate

Gel Filtration / Desalting

0045071	RoboColumn HW-40F	0.2 mL x 8 columns
0045072	RoboColumn HW-40F	0.6 mL x 8 columns

Ion Exchange Chromatography

0045027	RoboColumn Sulfate-650F	0.2 mL x 8 columns
0045028	RoboColumn Sulfate-650F	0.6 mL x 8 columns
0045023	RoboColumn GigaCap S-650S	0.2 mL x 8 columns
0045024	RoboColumn GigaCap S-650S	0.6 mL x 8 columns
0045001	RoboColumn GigaCap S-650M	0.2 mL x 8 columns
0045002	RoboColumn GigaCap S-650M	0.6 mL x 8 columns
0045025	RoboColumn GigaCap Q-650S	0.2 mL x 8 columns
0045026	RoboColumn GigaCap Q-650S	0.6 mL x 8 columns
0045003	RoboColumn GigaCap Q-650M	0.2 mL x 8 columns
0045004	RoboColumn GigaCap Q-650M	0.6 mL x 8 columns
0045005	RoboColumn GigaCap CM-650M	0.2 mL x 8 columns
0045006	RoboColumn GigaCap CM-650M	0.6 mL x 8 columns
0045007	RoboColumn GigaCap DEAE-650M	0.2 mL x 8 columns
0045008	RoboColumn GigaCap DEAE-650M	0.6 mL x 8 columns
0045021	RoboColumn NH2-750F	0.2 mL x 8 columns
0045022	RoboColumn NH2-750F	0.6 mL x 8 columns

Mixed Mode Chromatography

0045051	RoboColumn MX-Trp-650M	0.2 mL x 8 columns
0045052	RoboColumn MX-Trp-650M	0.6 mL x 8 columns
0045053	RoboColumn Ca⁺⁺Pure-HA	0.2 mL x 8 columns
0045054	RoboColumn Ca⁺⁺Pure-HA	0.6 mL x 8 columns

Hydrophobic Interaction Chromatography

0045031	RoboColumn Phenyl-600M	0.2 mL x 8 columns
0045032	RoboColumn Phenyl-600M	0.6 mL x 8 columns
0045033	RoboColumn Butyl-600M	0.2 mL x 8 columns
0045034	RoboColumn Butyl-600M	0.6 mL x 8 columns
0045035	RoboColumn PPG-600M	0.2 mL x 8 columns
0045036	RoboColumn PPG-600M	0.6 mL x 8 columns
0045037	RoboColumn Phenyl-650M	0.2 mL x 8 columns
0045038	RoboColumn Phenyl-650M	0.6 mL x 8 columns
0045089	RoboColumn Butyl-650M	0.2 mL x 8 columns
0045090	RoboColumn Butyl-650M	0.6 mL x 8 columns
0045091	RoboColumn Hexyl-650C	0.2 mL x 8 columns
0045092	RoboColumn Hexyl-650C	0.6 mL x 8 columns

Affinity Chromatography

0045061	RoboColumn AF-rProtein A-650F	0.2 mL x 8 columns
0045062	RoboColumn AF-rProtein A-650F	0.6 mL x 8 columns
0045063	RoboColumn AF-rProtein A HC-650F	0.2 mL x 8 columns
0045064	RoboColumn AF-rProtein A HC-650F	0.6 mL x 8 columns
0045065	RoboColumn AF-rProtein L-650F	0.2 mL x 8 columns
0045066	RoboColumn AF-rProtein L-650F	0.6 mL x 8 columns