Pre-packed Columns

How do I install SkillPak 1 mL and 5 mL columns?

SkillPak 1 mL and 5 mL columns are designed to be operated with commonly used low or medium pressure liquid chromatography systems.

If you encounter any challenge, contact your local Technical Support experts.

How reproducible are SkillPak columns?

Separation on SkillPak columns is highly reproducible, which is mandatory to allow you to use such columns during the development of your purification processes. As an example, a SkillPak 5 mL column pre-packed with TOYOPEARL AF-rProtein A HC-650F resin was loaded with 23.5 mg of mAb feedstock. After 10 cycles the average recovery was 91% (average yield was 21.3 mg) as shown in the table below. Both pH max for elution and yield were highly consistent throughout the 10 cycles.

What are SkillPak columns made of?

The hardware of SkillPak 1 mL and 5 mL columns is composed of bio-compatible polymers, mainly polypropylene. For detailed information about its composition, contact your local Technical Support experts.