General FAQs on purification

General FAQs on Purification

What is the difference between TOYOPEARL and TSKgel 5PW resins?

The TSKgel 5PW resins have the same polymer chemistry as Toyopearl resins and also the same post polymerization functionalization chemistry. The only difference is that the degree of crosslinking is higher which helps them withstand the higher back pressures generated by their smaller particle sizes. 

What is the stability data for TOYOPEARL and TSKgel resins?

Based on stability data, most Toyopearl and TSKgel resins have a proven shelf life of 10 years (for resin types launched less than 10 years ago, the stability data is only available starting the launch date!) without any adverse effects on performance.

What is the best method for packing TOYOPEARL and TSKgel resins?

One of our chromatography experts explained standard packing procedures for TOYOPEARL AF-rProtein L-650F during a Tosoh Tuesday webinar. Those procedures can be applied to most TOYOPEARL and TSKgel resins.

You can find detailed procedures for specific resins in our User Manual

Which buffer are the best suited to pack TOYOPEARL and TSKgel resins?

Packing buffers are depending on the chromatographic mode. You can find some standard buffers to start with in the Instruction Manual. You should then determine empirically which buffer is the most suitable for your set-up. As a basic rule, we recommend using the buffer with the highest viscosity in comparison with the ones that will be used in the chromatographic separation process.

How should I clean TOYOPEARL and TSKgel resins?

TOYOPEARL resins can be cleaned in the column or removed from the column and treated in bulk. The cleaning method and duration of treatment depend on the extent of contamination. At least three bed volumes and/or 20-30 min contact time of cleaning solution are typically employed in column washing procedures. See our User Manual for detailed procedures for the different TOYOPEARLS and TSKgel resins or contact your local Technical Support experts to discuss your needs.

What is the chemical stability of TOYOPEARL and TSKgel resins?

Sanitization or cleaning may be conducted with up to 1.0 mol/L NaOH or 1.0 mol/L HCI depending upon the ligand. Detailed information can be found in the respective Resin Information Sheets.

What is the mechanical stability of TOYOPEARL and TSKgel resins?

The polymeric backbone of TOYOPEARL and TSKgel resins permits high flow rates for maximum throughput and productivity. Most TOYOPEARL resins may be operated up to 6 bar and TSKgel PW-type resins may be operated up to 20 bar. These properties of the resins combined with the narrow particle size distributions result in superior pressure-flow characteristics for the packed TOYOPEARL bed. Linear velocities of 300 - 500 cm/h generate a pressure of between 1 and 2 bar in a 20 cm length bed. Achievement of high linear velocities at relatively low pressure enables high throughput production scale chromatography using equipment with moderate pressure limitations. Detailed information can be found in the respective Resin Information Sheets.

How is the typical packing pressure mentioned in the Resin Information Sheets and resin specifications measured?

The mentioned “typical packing pressure” is the pressure drop across the bed length, and not the system pressure. The latter sums up the pressure drop across the bed length plus the pressure generated by the hardware (frits, valves, tubings, detectors etc.)

How important are plate counts and asymmetry for the different chromatography modes?

Chrom. Mode Asymmetry Plate count
IEC Important Less important
AFC Important Not important
SEC Important Important
HIC Important Less Important (depending on molecular interaction with surface)
MXC Important Less important
Flow through mode Less important Less important

If you need assistance for the packing of your chromatography column,
don’t hesitate to contact your local Technical Support experts.