Tosoh
customer magazine
TSKgel UP-SW3000 features the same pore size as the well-established
TSKgel G3000SW
XL
. The calibration curve and the molecular weight range
of the new 2 μm TSKgel UP-SW3000 is similar to those of 5 µm TSKgel
G3000SW
XL
and 4 µm TSKgel SuperSW3000. Hence, methods developed
on conventional gel filtration columns can be easily transferred to UHPLC
technology by using TSKgel UP-SW.
TSKgel UP-SW is available in two column lengths; The shorter 15 cm
column can be used to speed up analysis and increase throughput while
maintaining resolution. The 30 cm column delivers dramatically increased
resolution e.g. between mAb fragments, monomers, and aggregates. The
use of the 15 cm column for aggregate analysis is described on page 5.
To achieve maximum resolution and fully exploit the potential of the
columns the use of a UHPLC system is highly recommended. A so-called
DC (direct connect) guard column can be attached directly to the analytical
column to minimize extra column band broadening while preventing early
deterioration of the analytical column.
TSKgel SW columns stand out from other silica-based high performance
size exclusion columns by virtue of their large pore volumes. They are
based on highly porous, ultra-pure silica particles, the surface of which
has been shielded from interacting with proteins by applying a proprietary
surface chemistry. This ensures highest recoveries and accurate quantita-
tion. The validated manufacturing and packing process delivers a reliable
batch-to-batch reproducibility.
03
What’s New
Columns
TSKgel UP-SW3000 Columns for UHPLC use
TSKgel UP-SW3000, the new 2 micron UHPLC column with 25 nm pore size, is the latest addition to the renowned TSKgel
SW series of silica based gel filtration columns. For decades, TSKgel G3000SW
XL
columns have been the gold stan-
dard for QC of therapeutic antibodies in Biopharma. Over 40 years of experience in developing and producing SEC
stationary phases are built into the new series of UHPLC columns for the analysis of proteins in the range of 10 to
500 kDa.
Figure :
Batch-to-batch Reproducibility for TSKgel UP-SW3000
Figure 1
Comparison of calibration curves
Protein Standard on TSKgel UP-SW3000 (red), TSKgel SuperSW3000 (blue) and
TSKgel G3000SW
XL
(grey)
4
6
8
10
12
14
elution volume [mL]
1
2
3
4
5
6
7
log (molar mass)
1
1‘
2‘
2
3
4
5
3
5
7
9
11
13
min
UV @ 280 nm
