Tosoh
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The projects submitted for the scholarship covered a broad range of
target molecules: from enzymes from recombinant and natural sour-
ces over ingredients of citrus fruits to biofilms. Josefine – a Ph.D.
student at the Institute of Process Engineering in Life Sciences, Bio-
molecular Separation Engineering (MAB), Karlsruhe Institute for Tech-
nology – proposed a project on purification of PEGylated proteins. Her
final target is to analyze the influence of polymer modifications on the
stability of proteins used in industrial applications.
The selected modification – called PEGylation – consists in the cova-
lent attachment of polyethylene glycol (PEG) to the target molecule. A
typical PEGylation reaction delivers a mixture of products that differ
either in the number of bound PEG chains (PEGamers) or in the posi-
tion of bound PEG molecules (isoforms). The PEGamers and isoforms
need to be separated in order to analyze their individual stability.
Liquid chromatography already proved to be a good approach to se-
parate PEGylated proteins, for preparative but also for analytical pur-
poses. Ion exchange and size exclusion chromatography will be ap-
plied in Josefine’s project first and we are happy to supply a portfolio
of TOYOPEARL GigaCap ion exchange media and HW size exclusion
media for this project. Chromatography experts from Tosoh Biosci-
ence will support Josefine throughout her projects through periodic
consultancy.
The prize and the certificate were handed over at the Chromatography
Workshop held in Stuttgart in September 2015.
AND THE WINNER IS:
JOSEFINE MORGENSTERN FROM KIT, KARLSRUHE
At HPLC 2015 in Geneva Tosoh Bioscience presented the new Tosoh Chromatography Scholarship. For this first editi-
on master and PhD students as well as post-docs had the opportunity to submit projects on purification of bio-
molecules. Out of the many applications submitted, Ms. Josefine Morgenstern was appointed as the winner of the
scholarship. It consists of a free participation in the renowned Tosoh Bioscience Chromatography Training in down-
stream processing and a starter package of chromatography media needed for the proposed project. The scholar-
ship was open to German-speaking students as this was the language of all three workshops in 2015.
06
TOSOH
SCHOLARSHIP
The Tosoh Grant 2016
will support an analytical application of
biochromatography.
