7
IEC
IEC
TSKgel STAT SERIES
TSKgel STAT columns are designed for high efficiency
separation of biomolecules and low molecular weight com-
pounds. They provide superior performance at reduced
analysis time. STAT columns are available in various for-
mats and sizes of the mono-disperse particles (5, 7 and
10 µm) to perfectly match specific application needs.
The surface of the hydrophilic non-porous particles con-
sists of an open access network of multi-layered ion ex-
change groups (carboxymethyl, sulfopropyl or quaternary
ammonium groups; see Figure 3). The innovative bonding
chemistry results in columns that show a reasonable sam-
ple capacity while traditional non-porous resins usually
show limited capacity due to lower surface area.
For fast and ultrafast IEC analysis short columns (3 mm ID x
3.5 cm length) are packed with large 10 µm particles. They
are ideally suited for rapid candidate screening or process
monitoring. Longer columns (4.6 mm ID x 10 cm length)
packed with 7 µm particles were designed for high reso-
lution IEC separation. They are perfect for the analysis of
nucleic acids, mAb variants, protein aggregates or PEGylat-
ed proteins. The DNA-STAT columns (4.6 mm ID x 10 cm
length) packed with 5 µm Q-type anion exchange resin are
optimized for the analysis of nucleic acids.
The relatively large particle sizes support fast separation at
moderate pressure while at the same time the proprietary
surface modification technology ensures a high density of
functional groups going along with high sample capacity.
Product Highlights TSKgel STAT series
Very efficient chromatography for high as well as low
MWsolutesmade possible by novel bonding chemistry
and the absence of micro-pores
High speed and high resolution analysis of
Biomolecules in HPLC and UHPLC
Higher adsorption capacities and lower pressures
compared with smaller particle sized TSKgel NPR
columns
7 or 10 µm particles (TSKgel Q-STAT) and 5 µm
particles (TSKgel DNA-STAT)
7 or 10 µm particles for SP and CM chemistries
Applications with TSKgel STAT Anion Exchange
Columns
Nucleotides
Mono-, di-, and tri-nucleotides were separated with ex-
cellent peak shape on a TSKgel DNA-STAT column. The
narrow, symmetrical peaks, as shown in Figure 4, demon-
strate the absence of micropores on this new generation of
non-porous resin columns. TSKgel DNA-STAT columns are
also, as the name implies, first choice for large nucleic acid
fragments.
Protein
Ionic group
Hydrophilic
chain
Non porous material
Schematic diagram of TSKgel stat series
figure 3
Retention time (min)
Column:
TSKgel DNA-STAT, 5µm, 4.6mm ID x 10cm
Eluent:
A: 20mmol/L Tris-HCl (pH8.5)
B: 0.75mol/L NaCl in buffer A
Gradient:
50% B (0min), 75% B (25min)
Flowrate:
0.8mL/min
UV@260nm (1Abs/1000mV)
C, A
G
U
95
85
75
65
55
45
35
25
15
5
0
5
10
15
20
25
2d-AMP
2d-CMP
TMP
2d-UMP
2d-GMP
2d-CDP
2d-ADP
TDP
2d-UDP
2d-GDP
2d-CTP
ATP
TTP
2d-UTP
2d-GTP
T
figure 4
Column: TSKgel DNA-STAT, 5 µm, 4.6 mm ID x 10.0 cm L;
Eluent: A: 20 mmol/L Tris-HCI (pH 8.5); B: 0.75 mol/L NaCl in buffer A;
Gradient: 50% B (0 min), 75% B (25 min); Flow rate: 0.8 mL/min;
Detection: UV @ 260 nm
High resolution separations of nucelotides
