11
IEC
IEC
TSKgel Bioassist SERIES
TSKgel BioAssist columns are based on methacrylate
particle design technology. TSKgel BioAssist Q contains
particles with very large pores (~400 nm) that are deriva-
tized with a network of polyamine groups. The capacity of
TSKgel BioAssist Q has been shown to be high over a
wide molecular weight range (up to 1,000,000 Da). TSKgel
BioAssist S is packed with particles possessing 130 nm
pores functionalized with sulfopropyl groups.
TSKgel BioAssist analytical IEC columns are provided in a
4.6 mm ID x 5 cm L PEEK housing with 7 μm or 10 μm par-
ticles for the respective S and Q functionalities.
Semipreparative TSKgel BioAssist columns are also avail-
able with a 13 μmparticle size packed in a 10 mm ID x 10 cm L
housing. The longer length of the semi-preparative column
compensates for the increased particle size, resulting in
similar resolution to the analytical column.
Product Highlights TSKgel BioAssist series
Pore structure and bonding chemistry of TSKgel
BioAssist Q/S columns provide high capacity for
small to very large MW proteins and nucleic acids.
TSKgel BioAssist Q/S is suitable for use in systems
that are designed for HPLC, laboratory or semi-
preparative applications
TSKgel BioAssist columns are packed in 4.6 mm ID
or 10 mm ID PEEK hardware. Other columns are
available in glass and stainless steel for analytical,
semi-preparative and preparative applications.
Especially designed for the separation of large biomole-
cules the very large pores of the TSKgel BioAssist columns
offer high capacity and resolution at a low column pres-
sure drop. The polymerization technique used to construct
these columns results in a homogenous distribution of ion
exchange groups without significantly reducing pore size.
APPLICATIONS WITH TSKgel BioAsisst ANION
EXCHANGE COLUMNS
Performance Enhancement on FPLC System
TSKgel BioAssist Q is suitable for use in systems that are
designed for laboratory or semi-preparative applications.
Figure 11 demonstrates the performance enhancement of
TSKgel BioAssist Q over a competitive product when oper-
ated side-by-side on an FPLC system.
Performance enhancement on FPLC system
BioAssist Q
Competitor Q
10
Minutes
5
15
mAU
mAU
1200
1180
1600
1140
1120
1100
1080
1060
1040
1200
1180
1600
1140
1120
1100
1080
1060
1040
0
Column:
TSKgel BioAssist Q, 4.6 mm ID x 5 cm (PEEK)
Competitor Q, 5.0 mm ID x 5 cm
Elution:
30 min linear gradient from 0 to 1 mol/L NaCl
in 20 mmol/L sodium phosphate pH 8.0
Flow Rate:
1.0 mL/min
Detection:
UV @ 280 nm
Sample:
1) conalbumin, i) ovalbumin impurity
2) ovalbumin, 3) trypsin inhibitor
1
i
2
3
1
i
2
3
Performance enhancement on fplc system
figure 11
Column: TSKgel BioAssist Q, 4.6 mm ID x 5 cm L (PEEK),
Competitor Q, 5.0 mm ID x 5 cm L; Elution: 30 in linear gradient from
0 to 1 mol/L NaCl in 20 m ol/L sodiu phosphate p 8.0; Flow Rate: 1.0 mL/
min; Detection: UV@ 280 nm; Sample: 1) conalbumin, i) ovalbumin impurity,
2) ovalbumin, 3) trypsin inhibitor
