13
IEC
IEC
TSKgel 5PW SERIES
The G5000 PW base resin of the TSKgel 5PW series is a
spherical particle with a mean pore size of 100 nanometer.
The chemistries of DEAE, SP and CM functionalities result
in standard ion exchangers while the chemistry employed
in the manufacturing of TSKgel SuperQ-5PW results in a
higher capacity strong anion exchanger by introducing
polyamine functional groups. Due to the higher density of
anion exchange sites TSKgel SuperQ-5PW has a smaller
effective pore size and a higher binding capacity than
TSKgel DEAE-5PW.
Applications with TSKgel 5PW Anion Exchange
Columns
Analysis of a synthetic Oligonucleotide on TSKgel SuperQ-
5PW
Figure 14 shows the analysis of a 16-mer morpholine oligo-
nucleotide on TSKgel SuperQ-5PW column using a NaCl
gradient in a 10 mmol/L sodium hydroxide mobile phase.
Analysis of high MW RNA on TSKgel DEAE-5PW
Figure 15 shows the fractionation of high molecular weight
E. coli RNA on TSKgel DEAE-5PW, effectively utilizing the
large 100 nm pores of this base resin.
Column:
TSKgel SuperQ-5PW, 7.5mm ID x 7.5cm
Sample:
16-mer morpholine oligonucleotide,
AAG AAG AAG AGG GGA G
Sample load:
0.5 O.D. (optical density)
Mobile phase:
A: 10mmol/L NaOH
B: 10mmol/L NaOH with 1mol/L NaCl
Gradient:
Initial: 0% B
40min: 50% B
41min: 100% B
46min: 100% B
Flow Rate:
1 mL/min
Detection:
UV @ 254nm
Analysis of synthetic oligonucleotide on TSKgel SuperQ-5PW
Minutes
40
30
20
10
0
analysis of synthetic oligonucleotide on TSKgel
SuperQ-5PW
Column: TSKgel SuperQ-5PW, 7.5 mm ID x 7.5 cm L;
Sample: 16-mer morpholine oligonucleotide, AAG AAG AAG AGG GGA G;
Sample load: 0.5 O.D. (optical density); Mobile phase: A: 10 mmol/L NaOH;
B: 10 mmol/L NaOH with 1 mol/L NaCl; Gradient: Initial: 0 % B, 40 min: 50 % B,
41 min: 100 % B, 46 min: 100% B; Flow rate: 1 mL/min; Detection: UV @ 254 nm
figure 14
Large pore TSKgel DEAE-5PW resolves high MW RNA
Minutes
0
40
80
4S tRNA
5S rRNA
16S rRNA
23S rRNA
High MW
Impurities
Column:
Sample:
Elution:
Flow Rate:
Detection:
TSKgel DEAE-5PW, 6mm ID x 15cm
total E. coli RNA
300min linear gradient from 0.3mol/L to 1.0mol/L NaCl
in 0.1mol/L Tris-HCl, pH 7.6
1.0mL/min
UV @ 260nm
large pore TSKgel DEAE-5PW resolves high MW RNA
Colu n: TSKgel DEAE-5PW, 6 mm ID x 15 cm L; Sample: total E. coli RNA;
Elution: 300 min linear gradient from 0.3 mol/L NaCl in 0.1 mol/L Tris-HCI,
pH 7.6; Flow rate:1. L/min; Detection: UV @ 26 n
figure 15
