sec
SEC
21
TSKgel PW and TSKgel PW
XL
columns -
Gel Filtration Chromatography of water soluble polymers
HIGHLIGHTS
Hydrophilic, rigid, spherical, porous methacrylate beads
pH range of 2 to 12, with up to 50% organic solvent
Temperatures up to 80°C (50°C for TSKgel G-DNA-PW)
Wide separation range up to 2 x 10
7
Da for linear polymers
Linear SEC column line incorporating proprietary multi-pore
technology
Specialty columns for low salt separation of cationic polymers
Polymeric TSKgel PW and high resolution TSKgel PW
XL
columns are
designed for SEC of water soluble organic polymers, polysaccharides,
DNA, and RNA. They are based on a hydrophilic polymethacrylate
matrix. The range of pore sizes in which TSKgel PW and TSKgel PW
XL
columns are available permits a wide spectrum of water soluble
substances to be analyzed. The properties and molar mass separation
ranges for all TSKgel PW series columns are summarized in
Table II
.
Stable from pH 2 to 12, TSKgel PW series columns can be used in mobile
phases of water or buffer (up to 20% methanol/80% aqueous) and can
tolerate temperatures up to 80 ˚C (50 ˚C for TSKgel G-DNA-PW column).
For analytical purposes the TSKgel PW
XL
columns are preferred
because of their higher resolution whereas for preparative work the
60 cm TSKgel PW columns are recommended because higher sample
amounts can be applied. For the analysis of proteins and peptides we
recommend to use silica based SW type columns.
A number of specialty columns include columns for oligosaccharides,
nucleic acids, and samples with a broad molecular weight range. A large
pore G6000PW phase is available in PEEK column hardware (TSKgel
BioAssist G6PW) for ultra-low sample adsorption during virus analysis.
TSKgel PW
XL
-CP columns are especially suited for the separation of
cationic polymers.
The latest additions to the TSKgel PW family are high resolution semi-
micro SEC columns: TSKgel SuperoligoPW for oligomer analysis and
TSKgel SuperMultiporePW columns for MW distribution analysis by
linear SEC. The packing material in the TSKgel SuperMultiporePW
columns is more hydrophilic than that of TSKgel PW
XL
columns, which
further reduces the risk of adsorption of hydrophilic polymers.
TSKgel Column
Particle size (μm)
Pore size (nm)
MW range
(PEG/PEO)
Dextrans*
Globular Proteins
G2000PW
12
12.5
< 2 x 10
3
-
< 5 x 10
3
G2500PW
12, 17
< 20
< 3 x 10
3
< 3 x 10
3
< 8 x 10
3
G3000PW
12, 17
20
< 5 x 10
4
< 6 x 10
4
5 x 10
2
- 8 x 10
5
G4000PW
17
50
< 3 x 10
5
1 x 10
3
- 7 x 10
5
1 x 10
4
- 1.5 x 10
6
G5000PW
17
100
< 1 x 10
6
5 x 10
4
- 2.5 x 10
6
< 1 x 10
8
G6000PW/ BioAssist G6PW
17
> 100
< 8 x 10
6
5 x 10
5
- 5 x 10
7
< 2 x 10
8
GMPW
17
< 10 - 100
5 x 10
2
- 8 x 10
6
< 5 x 10
7
< 2 x 10
8
G2500PW
XL
7
< 20
< 3 x 10
3
< 8 x 10
3
G3000PW
XL
7
20
< 5 x 10
4
< 6 x 10
4
5 x 10
2
- 8 x 10
5
G4000PW
XL
10
< 50
< 3 x 10
5
1 x 10
3
- 7 x 10
5
1 x 10
4
- 1.5 x 10
6
G5000PW
XL
10
100
< 1 x 10
6
5 x 10
4
- 2.5 x 10
6
< 1 x 10
8
G6000PW
XL
13
> 100
< 8 x 10
6
5 x 10
5
- 5 x 10
7
< 2 x 10
8
G-DNA-PW
10
> 100
< 8 x 10
6
< 5 x 10
7
GMPW
XL
13
10 - 100
5 x 10
2
- 8 x 10
6
< 5 x 10
7
< 2 x 10
8
G-Oligo-PW
7
12.5
0< 3 x 10
3
< 5 x 10
3
SuperMultiporePW-N
4
n/a
3 x 10
2
- 5 x 10
4
SuperMultiporePW-M
5
n/a
5 x 10
2
- 1 x 10
6
SuperMultiporePW-H
8 (6-10)
n/a
1 x 10
3
- 1 x 10
7
SuperOligoPW
3
n/a
1 x 10
2
- 3 x 10
3
G3000PW
XL
-CP
7
20
< 9 x 10
4
G5000PW
XL
-CP
10
100
< 1 x 10
6
G6000PW
XL
-CP
13
> 100
< 2 x 10
7
Column: TSKgel PW columns, 7.5 mm ID x 60 cm L; TSKgel PW
XL
, TSKgel PW
XL
-CP, G-Oligo-PW & G-DNA-PW, 7.8 mm ID x 30 cm L
Elution: Polyethylene glycols and oxides: distilled water; dextrans: 0.2 mol/L phosphate buffer, pH 6.8
Flow rate: 1.0 mL/min, except for TSKgel SuperMultiporePW and TSKgel SuperOligoPW columns: 0.6 mL/min
Note: *Maximum separation range determined from estimated exclusion limits
table II
Properties and separation ranges for TSKgel PW-type packings
