SEC
28
TSKgel ALPHA AND SuperAW GEL FILTRATION COLUMNS
Gel Filtration and Gel Permeation Chromatography of water soluble and polar organic-soluble polymers
HIGHLIGHTS
A unique hydrophilic, polymer resin is available in conventional
column dimensions (Alpha) and high throughput column format (Su-
perAW).
Exhibits strong mechanical stability and minimal swelling
characteristics
A wide range of solvent compatibility, from 100% water to 100% non-
polar organic solvents
The reduced particle size and shorter column length of
TSKgel SuperAW columns provide equivalent resolution in one half
the time for high throughput applications.
Unlike polystyrene-divinylbenzene (PS-DVB) resins that may adsorb
polymers due to hydrophobic interaction, both the TSKgel Alpha and
SuperAW columns allow for the separation of polymers soluble in
methanol.
Provide accurate molecular weight determination of samples in
dimethyl formamide and exhibit normal retention of polystyrene
polymers
System peaks from salts in the eluent elute away from
the oligomer of interest, providing accurate MW determinations.
Column Selection
The
TSKgel Alpha Series
consists of six columns with three particle
sizes: 7, 10, and 13 µm. These columns span a wide MW separation
range from 10
2
to more than 1 x 10
6
Da when using polyethylene
oxide (PEO) as a MW standard. Exclusion limits for the TSKgel Alpha
columns for polyethylene oxide (PEO), polyethylene glycols (PEG) and
polystyrenes (PS) are shown in the table below. Calibration curves
for the TSKgel Alpha Series columns are shown on the next page for
polyethylene oxide, polyethylene glycol and polystyrene standards.
The
TSKgel SuperAW series
contains a similar chemistry as the TSKgel
Alpha series but offers the benefit of smaller particle sizes (4 µm to 9
µm) and smaller column dimensions. Reductions in analysis time and
mobile phase consumption make SuperAW columns ideal for high
throughput applications. TSKgel Alpha and SuperAW columns are
offered in 5 discrete exclusion ranges and 1 mixed bed. Both column
types can accommodate polymer standards up to several million Dalton
molecular weight (see calibration curves on the next page
TSKgel Column
Particle size (µm)
Exclusion limit (Da) for various standards and eluents
PEO
a
/H
2
O
PS
b
/10 mmol/L LiBr
PEG
c
/10 mmol/L LiBr
in DMF
in MeOH
Alpha-2500
7
5 x 10
3
1 x 10
4
1 x 10
4
Alpha-3000
7
9 x 10
4
1 x 10
5
6 x 10
4
Alpha-4000
10
4 x 10
5
1 x 10
6
3 x 10
6
Alpha-5000
10
1 x 10
6
7 x 10
6
N.D.
Alpha-6000
13
> 1 x 10
7
> 1 x 10
7
N.D.
Alpha-M
13
> 1 x 10
7
> 1 x 10
7
N.D.
SuperAW2500
4
5 x 10
3
8 x 10
3
1 x 10
4
SuperAW3000
4
9 x 10
4
8 x 10
4
1 x 10
5
SuperAW4000
6
1 x 10
6
6 x 10
5
6 x 10
5
SuperAW5000
7
1 x 10
6
*
N.D.
N.D.
SuperAW6000
9
1 x 10
7
*
N.D.
N.D.
SuperAWM-H
9
1 x 10
7
*
N.D.
N.D.
N.D. = not determined a Polyethylene oxide b Polystyrene divinyl benzene c Polyethylene glycol
* Exclusion limit for SuperAW5000, SuperAW6000, and SuperAWM-H are estimated, respectively
table IV
Exclusion limits for TSKgel Alpha Series and SuperAW Series columns
