SEC
30
Applications of TSKgel ALPHA AND SuperAW Gel Filtration columns
The versatility of using TSKgel Alpha columns with various
polar solvents is illustrated in
Figure 24
for the analysis of
cellulose derivatives. A TSKgel Alpha-M column was used
to separate ethylcellulose with the polar solvent DMF and
ethylhydroxyethyl cellulose with methanol.
The separation of polyvinylalcohol with different degrees of
saponification is shown in
Figure 25
. This separation was performed
with a TSKgel Alpha-5000 and a TSKgel Alpha-3000 column in series
using a hexafluoroisopropanol mobile phase.
Figure 26
shows that the column efficiency of TSKgel SuperAW series
columns is maintained in a wide variety of polar organic solvents.
Column: TSKgel Alpha-5000 and Alpha-3000, 7.8 mm ID x 30 cm L in series
Sample: degree of saponification of polyvinyl alcohol: A. 75%, B. 88%,
C. 100%; Eluent: hexafluoroisopropanol (HFIP); Flow rate: 0.5 mL/min;
Temperature: 40°C; Detection: RI
Polyvinylalcohol characterization using TSK-GEL Alpha-5000
and Alpha-3000 columns in series
10
20
30
40
50
Minutes
l
:
Sa ple:
Eluent:
Flow Rate:
Temperature:
Detection:
TSKgel Alpha-5000 and Alpha-3000, 7.8mm ID x 30cm in s ries
degree of saponification f polyvinyl alc hol:
A. 75%; B. 88%; C. 100%
hexafluoroisopropanol (HFIP)
0.5mL/min
40°C
RI
A
B
C
figure 25
Polyvinylalcohol characterization using TSKgel Alpha-5000 and
Alpha-3000 columns in series
figure 24
TSKgel Alpha-M separation of cellulose derivatives
10
20
30
0
10
20
30
Minutes
A.
B.
mV
Column:
Sample:
Elution:
Flow Rate:
Temperature:
Detection:
TSKgel Alpha-M, 7.8mm ID x 30cm
A. 50µL ethylcellulose, 0.1%;
B. 50µL ethylhydroxyethylcellulose, 0.1%
A. 10mmol/L LiBr in DMF; B. 10mmol/L LiBr in methanol
0.5mL/min
40
°
C
RI
TSKgel Alpha-M separati n of cellulose derivatives
10
20
20
30
40
50
Minutes
mV
Column: TSKgel Alpha-M, 7.8 mm ID x 30 cm L;
Sample: A. 50 µL ethylcellulose, 0.1%; B. 50 µL ethylhydroxyethylcellulose,
0.1%; Elution: A. 1 mmol/L LiBr in DMF; B. 10 mmol/L LiBr in methanol;
Flow rate: 0.5 mL/min; Temperature: 40°C; Detection: RI
Solvent Compatibility of TSK-GEL SuperAW Series
Column:
TSK-GEL SuperAW Series (6.0 mm ID x 15 cm L)
Eluent:
Water
Flow rate:
0.6 mL/min
Temperature: 25
°
C
Detection:
Refractive index detector
Sample:
Ethylene glycol
Inj. volume:
5 µL (2.5 g/L)
0
5000
10000
15000
20000
25000
TP
HFIP
THF
DMSO
DMF
MeOH
MeOH/H
2
O=1/1
Original (H
2
O)
ACN
ACN/H
2
O=1/1
SuperAW2500
SuperAW3000
SuperAW4000
SuperAW5000
SuperAW6000
SuperAWM-H
figure 26
Solvent compatibility of TSKgel SuperAW series
Column: TSKgel SuperAW Series (6.0 mm ID x 15 cm L); Eluent: Water
Flow rate: 0.6 mL/min; Temperature: 25°C; Detection: Refractive index detector
Sample: Ethylene glycol; Inj. volume: 5 µL (2.5 g/L)
