SEC
36
Phthalate esters
Figure 30
demonstrates the high efficiency separation on a TSKgel
G1000H
XL
column for low molecular weight phthalate esters. Resolution
was close to baseline, even though the molecular weights of the esters
differed by less than 50 Da.
Phenol resin
The TSKgel GMH
XL
-L column has been designed to provide a complete
profile for high molecular weight samples that contain low molecular
weight additives. The calibration curve for this mixed-bed column
is shallow in the low molecular weight range of oligomers. Sample
adsorption is not observed.
Column: TSKgel G1000H
XL
, 7.8 mm ID x 30 cm L;
Sample: 1. polystyrene (10,200Da), 2. dioctylphthalate (391Da),
3. dibutylphthalate (278Da), 4.dipropylphthalate (250Da), 5. diethylphthalate
(222Da), 6. dimethylphthalate (194Da), 7. n-propylbenzene (120Da), 8.
ethylbenzene (116Da), 9. toluene (92Da), 10. benzene (78Da); Elution: THF;
Flow rate: 1.0 mL/min; Detection: UV @ 254 nm
For example, the complete profile of a phenol resin, with high resolution
of the low molecular weight components, is shown in
Figure 31
. Other
applications for the TSKgel GMH
XL
-L column include analyses of paint
materials, bond and adhesive components and synthetic polymer
additives.
Fatty acids
In
Figure 32
, two TSKgel G2000H
XL
columns in series separate a mixture
of fatty acids ranging from C4 to C30.
Column: TSKgel GMH
XL
-L, 7.8 mm ID x 30 cm L;
Sample: phenol resin; Elution: THF; Flow rate: 1.0 mL/min;
Detection: UV @ 254 nm
Column: TSKgel G2000H
XL
, two 7.8 mm ID x 30 cm L in series;
Sample: fatty acids; Elution: THF; Flow rate: 1.0 mL/min; Detection: RI
Applications of TSKgel H-TYPE Gel PERMEAtion columns
figure 31
Separation of phenol resin on TSKgel GMH
XL
-L
figure30
High resolution of phtalate ester on TSKgel G1000H
XL
High resolution of phthalate esters on TSKgel G1000H
XL
Minutes
Column:
Sample:
Elution:
Flow Rate:
Detection:
TSKgel G1000H
XL
, 7.8mm ID x 30cm
1. polystyrene (10,200Da), 2. dioctylphthalate (391Da),
3. dibutylphthalate (278Da), 4.dipropylphthalate (250Da),
5. diethylphthalate (222Da), 6. dimethylphthalate (194Da),
7. n-propylbenzene (120Da), 8. ethylbenzene (116Da),
9. toluene (92Da), 10. benzene (78Da)
THF
1.0mL/min
UV @ 254nm
10
5
1
2
3
4
5
6
7
8
9
10
Separation of phenol resin on TSKgel GMH
XL
-L
Minutes
Column:
Sample:
Elution:
Flow Rate:
Detection:
TSKgel GMH
XL
-L, 7.8mm ID x 30cm
phenol resin
THF
1.0mL/min
UV @ 254nm
10
5
0
figure 32
Separation of fatty acid
Separation of fatty acids
TSKgel G2000H
XL
, two 7.8mm ID x 30cm in series
fatty acids
THF
1.0mL/min
RI
Column:
Sample:
Elution:
Flow Rate:
Detection:
C
30
C
26
C
22
C
20
C
18
C
16
C
14
C
12
C
10
C
8
C
4
C
6
20
30
Minutes
