Iec
IEC
45
TSKgel BioAssist
columns are also based on methacrylate particle
design technology. TSKgel BioAssist Q contains particles with very
large pores (~400 nm) that are derivatized with a network of polyamine
groups. The capacity of TSKgel BioAssist Q has been shown to
be high over a wide molecular weight range (up to 1,000,000 Da).
TSKgel BioAssist S is packed with particles possessing 130 nm pores
functionalized with sulfopropyl groups. TSKgel BioAssist analytical
IEC columns are provided in a 4.6 mm ID x 5 cm L PEEK housing with 7
µm or 10 µm particles for the respective S and Q functionalities. Semi-
preparative TSKgel BioAssist columns are also available with a 13 µm
particle size packed in a 10 mm ID x 10 cm L housing. The longer length
of the semi-preparative column compensates for the increased particle
size, resulting in similar resolution to the analytical column.
The methacrylate chemistry also forms the backbone of non-porous
resin columns such as
TSKgel STAT
and
NPR
columns. Since rate-
limiting pore difusion is eliminated with nonporous particles, analysis
time is often reduced by as much as 80 % without loss in resolution.
Also, recoveries are routinely greater than 90 %.
TSKgel STAT ion exchange columns are packed with 5, 7 or 10 μm
hydrophilic non-porous resin particles of which the surface consists
of an open access network of multi-layered ion exchange groups
(carboxymethyl, sulfopropyl, or quaternary ammonium groups; see
Figure 1
.
These relatively large particle sizes, combined with an innovative
bonding chemistry, result in columns that enable fast equilibration
and analysis of complex biomolecular samples, attributes not found in
traditional mono-disperse, non-porous stationary phases.
Specific application needs are addressed by offering various column
formats and particle sizes: For fast and ultra-fast analysis (e.g. screening
or process monitoring) short 3 mm ID columns are packed with 10 µm
particles. For high resolution separations longer columns with 4.6 mm ID
are packed with 7 µm particles. The DNA-STAT column is packed with
smaller particles (5 µm).
TSKgel DEAE-NPR
,
SP-NPR
and
DNA-NPR
are packed with 2.5 µm
particles. High column efficiency coupled with low sample capacity
restricts theapplicationof thesecolumns to fast analysisandmicro-scale
preparative isolation. The DNA-NPR column is a longer version of the
DEAE-NPR column that allows improved resolution of oligonucleotides,
including those amplified by PCR. Small guard columns are available to
protect the DNA-NPR and DEAE-NPR columns.
In the development of new drug candidates, it is often desirable to use
the same backbone chemistry throughout the development process. For
that reason, the backbone of the 20 µm and 30 µm particle size TSKgel
PW-type resins and the larger particle size TOYOPEARL process media
are chemically similar to that used in prepacked TSKgel PW-type column
lines. As a result, TSKgel SuperQ-5PW scales directly to TOYOPEARL
SuperQ-650. Similarly, the TSKgel DEAE-5PW scales directly to TSKgel
DEAE-5PW bulk resins, which in turn scales to TOYOPEARL DEAE-650.
The same is true for CM and SP products in the cation exchange column
line.
figure 1
Schematic diagram of TSKgel STAT Series
Protein
Ionic group
Hydrophilic
chain
Non porous material
INTRODUCTION TO TSKgel ION EXCHANGE COLUMNS
